Increase in resistance to extended-spectrum cephalosporins in Salmonella isolated from retail chicken products in Japan.
Increase in resistance to extended-spectrum cephalosporins in Salmonella isolated from retail chicken products in Japan.
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DOI：
10.1371/journal.pone.0116927
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Fujimoto S
中科院分区：
文献类型：
--
作者：
Noda T;Murakami K;Etoh Y;Okamoto F;Yatsuyanagi J;Sera N;Furuta M;Onozuka D;Oda T;Asai T;Fujimoto S
产广谱β-内酰胺酶（ESBL）沙门氏菌是发达国家最重要的公共卫生问题之一。产esbls沙门氏菌菌株已在邻近日本的亚洲国家从人类身上分离出来，同时也分离出含有质粒介导的广谱头孢菌素（ESC）耐药基因ampC （pAmpC）的菌株。然而，只有少数研究调查了日本鸡肉产品中esc耐药沙门氏菌的流行情况，这是沙门氏菌传播的主要媒介。本研究的目的是调查日本鸡肉产品中产生esbl、携带pampc或耐碳青霉烯沙门氏菌的流行情况。在1996年至2010年期间收集的779份鸡肉产品样本中，总共有355份（45.6%）含有沙门氏菌，共分离出378种不同的菌株。在这些分离株中，对373株进行了ESCs、头孢霉素或碳青霉烯类耐药检测。然后通过PCR和DNA序列分析检测对一种或多种抗菌素耐药的分离株是否存在bla CMY、bla CTX-M、bla TEM和bla SHV耐药基因。共检测到35株耐药菌株，其中含有pAmpC （bla CMY-2）的菌株26株，含有bla CTX-M （n = 4，包含2个bla CTX-M-2和2个bla CTX-M-15基因）、bla TEM （n = 4，包含1个bla TEM-20和3个bla TEM-52基因）和bla SHV （n = 1，包含bla SHV-12）的产esbl菌株9株。含pampc和产esbl的沙门氏菌分离株均来自2005年以后采集的样本，耐药菌比例从2004年的0%显著增加到2010年的27.9%（趋势P = 0.006）。这种增加的部分原因是肠道沙门氏菌亚种数量的增加。婴儿肠血清型菌株含有约280 kb的质粒，其中含有靠近ISEcp1的bla CMY-2。含有质粒介导的bla CMY-2的esc耐药沙门氏菌在鸡肉产品中的传播表明，为了公共卫生的利益，需要制定持续监测策略。
Extended-spectrum β-lactamase (ESBL)-producing Salmonella are one of the most important public health problems in developed countries. ESBL-producing Salmonella strains have been isolated from humans in Asian countries neighboring Japan, along with strains harboring the plasmid-mediated extended-spectrum cephalosporin (ESC)-resistance gene, ampC (pAmpC). However, only a few studies have investigated the prevalence of ESC-resistant Salmonella in chicken products in Japan, which are the main vehicle of Salmonella transmission. The aim of this study was to investigate the prevalence of ESBL-producing, pAmpC-harboring, or carbapenem-resistant Salmonella in chicken products in Japan. In total, 355 out of 779 (45.6%) chicken product samples collected from 1996–2010 contained Salmonella, resulting in 378 distinct isolates. Of these isolates, 373 were tested for resistance to ESCs, cephamycins, or carbapenems. Isolates that showed resistance to one or more of these antimicrobials were then examined by PCR and DNA sequence analysis for the presence of the bla CMY, bla CTX-M, bla TEM, and bla SHV resistance genes. Thirty-five resistant isolates were detected, including 26 isolates that contained pAmpC (bla CMY-2), and nine ESBL-producing isolates harboring bla CTX-M (n = 4, consisting of two bla CTX-M-2 and two bla CTX-M-15 genes), bla TEM (n = 4, consisting of one bla TEM-20 and three bla TEM-52 genes), and bla SHV (n = 1, bla SHV-12). All pAmpC-harboring and ESBL-producing Salmonella isolates were obtained from samples collected after 2005, and the percentage of resistant isolates increased significantly from 0% in 2004 to 27.9% in 2010 (P for trend = 0.006). This increase was caused in part by an increase in the number of Salmonella enterica subsp. enterica serovar Infantis strains harboring an approximately 280-kb plasmid containing bla CMY-2 in proximity to ISEcp1. The dissemination of ESC-resistant Salmonella containing plasmid-mediated bla CMY-2 in chicken products indicates the need for the development of continuous monitoring strategies in the interests of public health.
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影响因子：
4.9
作者：
Izumiya, H;Mori, K;Watanabe, H
通讯作者：
Watanabe, H
影响因子：
9.4
作者：
Leinberger, Dirk M.;Grimm, Verena;Bachmann, Till T.
通讯作者：
Bachmann, Till T.
影响因子：
5.2
作者：
Mataseje, L. E.;Xiao, J.;Mulvey, M. R.
通讯作者：
Mulvey, M. R.
影响因子：
2.2
作者：
Murakami, Koichi;Nada, Tamie;Fujimoto, Shuji
通讯作者：
Fujimoto, Shuji
影响因子：
2.1
作者：
Ishihara K;Takahashi T;Morioka A;Kojima A;Kijima M;Asai T;Tamura Y
通讯作者：
Tamura Y