Studies of canine and feline sperm viability under different storage procedures
Studies of canine and feline sperm viability under different storage procedures
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不同储存程序下犬和猫精子活力的研究
DOI：
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Ulrika Hermansson
中科院分区：
文献类型：
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作者：
Ulrika Hermansson
精子的冷却和冷冻允许遗传物质的远距离运输和交换。冷冻精子可以保存更长的时间，有助于扩大濒临灭绝的犬科动物和猫科动物以及家养狗和猫的基因库。本研究对狗和猫精子的不同保存方法进行了评价。对冷冻前冷藏1-2天的狗精子存活率进行评估。结果表明，解冻后精子活力、质膜完整性和顶体完整性均无明显下降。研究结果表明，狗主人有可能在离家较近的地方收集精液，并将其冷冻后送到精子库进行冷冻和长期储存。在猫中，我们研究了冷休克的易感性和用于冷藏的膨化剂中蛋黄的有益作用。结果表明，猫附睾和电精精子均不受冷激的影响，添加20%的卵黄有利于猫附睾和电精精子的活力和顶体的完整性。电射精精子在活力、精子质膜完整性和顶体完整性方面优于来自同一雄猫的附睾精子。采用透明带结合试验（ZBA）测定了狗和猫的精子与透明带的结合能力。在狗身上，目的是测试不同的卵母细胞池的精子结合能力是否不同，以及是否需要1小时或4小时的共孵育。结果表明，在进行ZBA时应考虑卵母细胞的变化，并且精子和卵母细胞共孵育1小时对于新鲜和冷藏精子似乎足够，但对于冻融（FT）精子则不够，具体取决于所使用的冷冻介质。还对猫进行了ZBA试验，以确定FT卵母细胞是否可以使用（就像在狗身上一样），以及它们是否具有与新鲜体外成熟（IVM）卵母细胞相同的结合能力。由此可见，FT卵母细胞不适合用于猫的ZBAs。
Chilling and freezing of spermatozoa permits transport and exchange of genetic material over distance. Frozen spermatozoa, which can be preserved over longer periods, can contribute to widening the gene pools of endangered canids and felids, as well as of domestic dogs and cats. In the present study different storage methods for dog and cat spermatozoa were evaluated. The viability of dog spermatozoa that had been cold stored for 1–2 days prior to freezing was assessed. The results revealed that there was no significant deterioration in motility, sperm plasma membrane integrity or acrosome integrity post-thaw. The results suggest that it would be possible for dog owners to collect semen nearer home and send it chilled to a semen bank for freezing and long-term storage. In the cat, we investigated susceptibility to cold shock and the beneficial effects of egg yolk in the extender used for cold storage. The results of these studies indicate that neither epididymal nor electroejaculated cat spermatozoa are susceptible to cold shock, and that 20% egg yolk in the extender is beneficial for motility and acrosome integrity in epididymal and electro-ejaculated cat spermatozoa. Electroejaculated spermatozoa proved superior to epididymal spermatozoa from the same tomcats in terms of motility, sperm plasma membrane integrity and acrosome intactness. The sperm-zona binding capacity of dog and cat zona pellucidae (ZPs) was tested using a zona pellucida binding assay (ZBA). In the dog the aim was to test whether different oocyte pools differed in their sperm-binding capacity, and whether 1- or 4-hour co-incubation was required. The results revealed that variation in oocytes should be taken into account when performing the ZBA, and that 1-hour co-incubation of spermatozoa and oocytes appears to be sufficient for fresh and chilled spermatozoa, but not for frozen-thawed (FT) spermatozoa, depending on the freezing medium used. A ZBA for cats was also performed to determine whether FT oocytes could be used (as in the dog) and whether they have equal binding capacity to fresh, in vitro-matured (IVM) oocytes. It was concluded that FT oocytes are not suitable for ZBAs in the cat.
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DOI：
10.1071/rd9950871
发表时间：
1995
期刊：
Reproduction, fertility, and development
影响因子：
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作者：
P. Watson
通讯作者：
P. Watson
透明带穿刺可增强正常精子和畸精子家猫的精子对透明带的渗透。
影响因子：
--
作者：
Roth,TL;Howard,J;Wildt,DE
通讯作者：
Wildt,DE
正常精子与畸形精子供体的家猫（Felis catus）精子对寒冷引起的顶体损伤的敏感性。
影响因子：
3.6
作者：
Pukazhenthi,B;Pelican,K;Wildt,D;Howard,J
通讯作者：
Howard,J
顶体完整和顶体反应的人类精子可以启动与透明带的结合。
影响因子：
2.7
作者：
Morales,P;Cross,NL;Overstreet,JW;Hanson,FW
通讯作者：
Hanson,FW