Infrared reflection-absorption spectroscopy: principles and applications to lipid-protein interaction in Langmuir films.
Infrared reflection-absorption spectroscopy: principles and applications to lipid-protein interaction in Langmuir films.
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DOI：
10.1016/j.bbamem.2009.11.024
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发表时间：
2010-04
影响因子：
3.4
通讯作者：
Flach, Carol R.
中科院分区：
文献类型：
--
作者：
Mendelsohn, Richard;Mao, Guangru;Flach, Carol R.
关键词：
红外反射吸收光谱(IRRAS)在空气/水界面原位检测脂质/蛋白质单层膜，可从膜组分中获得独特的分子结构和取向信息。因此，这项技术非常适合于研究生理相关环境中的脂质/蛋白质相互作用。首先，描述了IRRAS实验的性质，并概述了可能获得的分子结构信息。随后，综述了几种类型的应用，包括脂链构象和倾斜度的测定以及蛋白质二级结构的阐明。目前的文章试图让读者了解IRRAS仪器的当前能力，以及迄今通过IRRAS对脂质、蛋白质和逐渐增加的复杂性的脂质/蛋白质膜进行的IRRAS研究所取得的结果的类型。最后，简要讨论了该技术可能的扩展。
Infrared reflection-absorption spectroscopy (IRRAS) of lipid/protein monolayer films in situ at the air/water interface provides unique molecular structure and orientation information from the film constituents. The technique is thus well suited for studies of lipid/protein interaction in a physiologically relevant environment. Initially, the nature of the IRRAS experiment is described and the molecular structure information that may be obtained is recapitulated. Subsequently, several types of applications, including the determination of lipid chain conformation and tilt as well as elucidation of protein secondary structure are reviewed. The current article attempts to provide the reader with an understanding of the current capabilities of IRRAS instrumentation and the type of results that have been achieved to date from IRRAS studies of lipids, proteins and lipid/protein films of progressively increasing complexity. Finally, possible extensions of the technology are briefly considered.
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影响因子：
2.9
作者：
Bi, XH;Taneva, S;Flach, CR
通讯作者：
Flach, CR
DOI：
10.1039/ft9969200525
发表时间：
1996-02-21
期刊：
JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS
影响因子：
--
作者：
Blaudez, D;Turlet, JM;Desbat, B
通讯作者：
Desbat, B
影响因子：
3.3
作者：
Brauner, JW;Flach, CR;Mendelsohn, R
通讯作者：
Mendelsohn, R
影响因子：
3.3
作者：
Dyck, Martina;Kerth, Andreas;Losche, Mathias
通讯作者：
Losche, Mathias
影响因子：
3.4
作者：
Gericke, A;Flach, CR;Mendelsohn, R
通讯作者：
Mendelsohn, R