Age-related changes in cyclic phosphatidic acid-induced hyaluronic acid synthesis in human fibroblasts
Age-related changes in cyclic phosphatidic acid-induced hyaluronic acid synthesis in human fibroblasts
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环磷脂酸诱导的人成纤维细胞透明质酸合成的年龄相关变化
DOI：
10.1007/s13577-017-0185-7
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发表时间：
2017
期刊：
影响因子：
4.3
通讯作者：
Murakami-Murofushi Kimiko
中科院分区：
文献类型：
--
作者：
Sano Katsura;Gotoh Mari;Dodo Kyoko;Tajima Noriaki;Shimizu Yoshibumi;Murakami-Murofushi Kimiko
透明质酸是细胞外基质的主要成分，对皮肤水合作用很重要。由于衰老导致皮肤脱水，我们的目的是阐明透明质酸相关蛋白在来自不同年龄（范围0.7-69岁）供体的人皮肤成纤维细胞中的mRNA表达。以前，我们报道了环磷脂酸（cPA），一种独特的磷脂介质，刺激表达的HAS 2和增加透明质酸合成的人皮肤成纤维细胞（供体年龄：3天）。在这项研究中，我们测量了透明质酸相关蛋白的mRNA表达：透明质酸合成酶（HAS）1-3，透明质酸酶-1，2和透明质酸结合蛋白（多功能蛋白聚糖）。此外，我们测试了cPA是否可以增加来自不同年龄供体的皮肤成纤维细胞中的透明质酸合成。HAS 1、3、透明质酸酶-1和-2的表达不随增龄而改变。而versican mRNA的表达则随增龄而下降。尽管认为真皮中透明质酸的量随着衰老而减少，但HAS 2的mRNA表达增加。但成纤维细胞分泌的透明质酸量并不随年龄增长而增加。这表明HAS 2的活性和/或蛋白表达随着衰老而降低。此外，我们观察到cPA在任何年龄都引起透明质酸合成的增加，并且这种作用随着年龄的增长而增加。这些结果表明，衰老使成纤维细胞对cPA治疗更加敏感。因此，cPA代表了通过增加真皮中透明质酸水平来维持和改善皮肤健康的合适候选物。
Hyaluronic acid is a major component of the extracellular matrix, which is important for skin hydration. As aging brings skin dehydration, we aimed to clarify the mRNA expression of hyaluronic acid-related proteins in human skin fibroblasts from donors of various ages (range 0.7–69 years). Previously, we reported that cyclic phosphatidic acid (cPA), a unique phospholipid mediator, stimulated the expression ofHAS2and increased hyaluronic acid synthesis in human skin fibroblasts (donor age: 3 days). In this study, we measured the mRNA expression of hyaluronic acid-related proteins: hyaluronan synthase (HAS) 1–3, hyaluronidase-1, -2, and hyaluronic acid-binding protein (versican). In addition, we tested whether cPA could increase hyaluronic acid synthesis in skin fibroblasts derived from donors of various ages. The expression ofHAS1,3, hyaluronidase-1, and -2 did not change with aging. However, the mRNA expression of versican decreased with aging. Although it is thought that the amount of hyaluronic acid in the dermis decreases with aging, the mRNA expression ofHAS2was increased. But the amount of hyaluronic acid secreted by fibroblasts did not increase with aging. This suggests that the activity and/or protein expression of HAS2 decrease with aging. Furthermore, we observed that cPA caused the increase of hyaluronic acid synthesis at any age, and this effect was increased with aging. These results suggest that aging made the fibroblasts more sensitive to cPA treatment. Therefore, cPA represents a suitable candidate for the health maintenance and improvement of the skin by increasing the level of hyaluronic acid in the dermis.
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一种新型脂质介质——环磷脂酸（cPA）及其生物学功能
影响因子：
5.2
作者：
K. MURAKAMI‐MUROFUSHI;M. Mukai;Susumu Kobayashi;Tetsuyuki Kobayashi;G. Tigyi;H. Murofushi
通讯作者：
H. Murofushi
DOI：
10.4161/derm.21923
发表时间：
2012-07-01
期刊：
Dermato-endocrinology
影响因子：
--
作者：
Papakonstantinou E;Roth M;Karakiulakis G
通讯作者：
Karakiulakis G
用从多头绒泡菌的粘变形虫中分离出的新型溶血磷脂酸 (PHYLPA) 抑制真核 DNA 聚合酶 α。
影响因子：
4.8
作者：
Kimiko Murakami;Masaki ShiodaB;Kazuhiko Kajiv;Shonen Yoshidall;H. Murofushi
通讯作者：
H. Murofushi
DOI：
10.1016/j.bbalip.2014.05.004
发表时间：
2014-09-01
影响因子：
4.8
作者：
Maeda-Sano, Katsura;Gotoh, Mari;Murakami-Murofushi, Kimiko
通讯作者：
Murakami-Murofushi, Kimiko
影响因子：
3.3
作者：
Gotoh M;Nagano A;Tsukahara R;Murofushi H;Morohoshi T;Otsuka K;Murakami-Murofushi K
通讯作者：
Murakami-Murofushi K