Detection of SARS-CoV-2 RNA in hospital wastewater from a low COVID-19 disease prevalence area.
Detection of SARS-CoV-2 RNA in hospital wastewater from a low COVID-19 disease prevalence area.
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DOI：
10.1016/j.scitotenv.2020.143226
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发表时间：
2021-02-10
期刊：
影响因子：
--
通讯作者：
Paragi M
中科院分区：
文献类型：
--
作者：
Gonçalves J;Koritnik T;Mioč V;Trkov M;Bolješič M;Berginc N;Prosenc K;Kotar T;Paragi M
以前关于SARS-CoV和MERS-CoV的研究报告称，在有症状和无症状的人的粪便中都检测到病毒RNA。这些临床观察表明，受影响社区的市政和医院废水可能含有SARS-CoV-2RNA。最近的研究也报道了在人类粪便中存在SARS-CoV-2RNA。基于废水的流行病学(WBE)是一种很有希望的方法来了解病毒在给定的集水区人群中的流行情况，因为废水中含有来自有症状和无症状个人的病毒。目前的研究报告了斯洛文尼亚首次在未经处理的废水中检测到SARS-CoV-2RNA。测试了两种尺寸的离心式过滤器：30 kDa和10Kda AMICON®Ultra-15离心式过滤器，其中10KDA的离心式过滤器具有更高的浓缩系数和更高的回收效率。对医院污水的监测结果表明，该方法可用于−19的监测，并可作为一种潜在的补充工具应用于城市污水处理厂的人群健康监测。斯洛文尼亚第一个报告在未经处理的废水中检测到SARS-CoV-2的研究。66.7%(10/15)未经处理的医院废水样本至少有一项SARS-CoV-2 RT-qPCR检测呈阳性。仅有一名新冠肺炎患者住院时，在未经处理的医院废水中检测到SARS-CoV-2RNA。
Previous studies on SARS-CoV and MERS-CoV reported the detection of viral RNA in the stool of both symptomatic and asymptomatic individuals. These clinical observations suggest that municipal and hospital wastewater from affected communities may contain SARS-CoV-2 RNA. Recent studies have also reported the presence of SARS-CoV-2 RNA in human feces. Wastewater-based epidemiology (WBE) is a promising approach to understand the prevalence of viruses in a given catchment population, as wastewater contains viruses from symptomatic and asymptomatic individuals. The current study reports the first detection of SARS-CoV-2 RNA in untreated wastewater in Slovenia. Two sizes of centrifugal filters were tested: 30 kDa and 10 kDA AMICON® Ultra-15 Centrifugal Filters, where 10 kDA resulted in a higher concentration factor and higher recovery efficiency. The results in hospital wastewater show that WBE can be used for monitoring COVID −19 and could be applied in municipal wastewater treatment plants as a potential complementary tool for public health monitoring at population level. The first study in Slovenia that reports the detection of SARS-CoV-2 in untreated wastewater. 66.7% (10/15) of untreated hospital wastewater samples tested positive for at least one SARS-CoV-2 RT-qPCR target. SARS-CoV-2 RNA was detected in untreated hospital wastewater when only one COVID-19 patient was hospitalized.
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DOI：
10.1016/j.coesh.2020.01.006
发表时间：
2020-08-01
影响因子：
--
作者：
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通讯作者：
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DOI：
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发表时间：
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期刊：
The Journal of infectious diseases
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作者：
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