Phylum Verrucomicrobia representatives share a compartmentalized cell plan with members of bacterial phylum Planctomycetes.
Phylum Verrucomicrobia representatives share a compartmentalized cell plan with members of bacterial phylum Planctomycetes.
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DOI：
10.1186/1471-2180-9-5
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发表时间：
2009-01-08
期刊：
影响因子：
4.2
通讯作者：
Fuerst JA
中科院分区：
文献类型：
--
作者：
Lee KC;Webb RI;Janssen PH;Sangwan P;Romeo T;Staley JT;Fuerst JA
Verrucomicrobia门是细菌领域的一个分支门，包括土壤、淡水和海水的微生物群落成员；最近，人们发现来自温泉的极端嗜酸物质可以氧化甲烷。至少有一个属，假杆菌，包括与编码真核小管蛋白的基因同源的物种。Verrucomicrobia与植物菌门成员具有独特的区隔细胞计划，而衣原体门成员包括具有复杂细胞内生命周期的人类病原体，已经提出了一个重要的超门关系。基于假设的超门关系，我们假设plantomycetes和Verrucomicrobia这两个独立的门的成员可能具有与经典原核生物组织不同的相似的超微结构计划。采用高压冷冻和低温替代技术，对疣菌门4个成员——棘疣菌（Verrucomicrobium spinosum）、dejongei假杆菌（prothecobacter dejongeii）、黄硫杆菌（Chthoniobacter flavus）和Ellin514菌株的细胞超微结构进行了观察。Verrucomicrobia门的这四个成员，代表了门内3个类水平的分支，被发现具有类似于plantomycetes门的区隔细胞计划。与所研究的所有planticomyetes一样，它们具有一个主要的pirellusomal室，其中包含被胞质内膜（ICM）包围的浓缩样核和核糖体，以及ICM和细胞质膜之间无核糖体的副质室。到目前为止，在领域细菌中发现的独特的区隔细胞计划仅在植物门中发现，并且挑战了我们原核生物细胞计划的概念，现在在领域细菌的第二个门中发现，在Verrucomicrobia门的成员中。因此，plantomomytes细胞计划至少出现在细菌的两个不同的门中，从其他证据表明，这些门在系统发育上与所提出的PVC （plantomyetes - verrucomicrobiia - chlamydiae）超门有关。在疣菌属的6个分支中，至少有3个分支存在这种细胞结构，这表明疣菌门的共同祖先也被划分并具有这种结构。在植物门和疣菌门中都存在这种区隔化的细胞计划，这表明这些门的最后共同祖先也是区隔化的。
The phylum Verrucomicrobia is a divergent phylum within domain Bacteria including members of the microbial communities of soil and fresh and marine waters; recently extremely acidophilic members from hot springs have been found to oxidize methane. At least one genus, Prosthecobacter, includes species with genes homologous to those encoding eukaryotic tubulins. A significant superphylum relationship of Verrucomicrobia with members of phylum Planctomycetes possessing a unique compartmentalized cell plan, and members of the phylum Chlamydiae including human pathogens with a complex intracellular life cycle, has been proposed. Based on the postulated superphylum relationship, we hypothesized that members of the two separate phyla Planctomycetes and Verrucomicrobia might share a similar ultrastructure plan differing from classical prokaryote organization. The ultrastructure of cells of four members of phylum Verrucomicrobia – Verrucomicrobium spinosum, Prosthecobacter dejongeii, Chthoniobacter flavus, and strain Ellin514 – was examined using electron microscopy incorporating high-pressure freezing and cryosubstitution. These four members of phylum Verrucomicrobia, representing 3 class-level subdivisions within the phylum, were found to possess a compartmentalized cell plan analogous to that found in phylum Planctomycetes. Like all planctomycetes investigated, they possess a major pirellulosome compartment containing a condensed nucleoid and ribosomes surrounded by an intracytoplasmic membrane (ICM), as well as a ribosome-free paryphoplasm compartment between the ICM and cytoplasmic membrane. A unique compartmentalized cell plan so far found among Domain Bacteria only within phylum Planctomycetes, and challenging our concept of prokaryote cell plans, has now been found in a second phylum of the Domain Bacteria, in members of phylum Verrucomicrobia. The planctomycete cell plan thus occurs in at least two distinct phyla of the Bacteria, phyla which have been suggested from other evidence to be related phylogenetically in the proposed PVC (Planctomycetes-Verrucomicrobia-Chlamydiae) superphylum. This planctomycete cell plan is present in at least 3 of 6 subdivisions of Verrucomicrobia, suggesting that the common ancestor of the verrucomicrobial phylum was also compartmentalized and possessed such a plan. The presence of this compartmentalized cell plan in both phylum Planctomycetes and phylum Verrucomicrobia suggest that the last common ancestor of these phyla was also compartmentalized.
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影响因子：
2.8
作者：
Lindsay, MR;Webb, RI;Fuerst, JA
通讯作者：
Fuerst, JA
影响因子：
3.2
作者：
Eltsov, M;Dubochet, J
通讯作者：
Dubochet, J
DOI：
10.1073/pnas.012516899
发表时间：
2002-12-24
影响因子：
11.1
作者：
Jenkins, C;Samudrala, R;Staley, JT
通讯作者：
Staley, JT
DOI：
10.1099/00221287-143-3-739
发表时间：
1997-03-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Lindsay, MR;Webb, RI;Fuerst, JA
通讯作者：
Fuerst, JA
影响因子：
3.2
作者：
HOBOT, JA;VILLIGER, W;KELLENBERGER, E
通讯作者：
KELLENBERGER, E