CD38 Is Robustly Induced in Human Macrophages and Monocytes in Inflammatory Conditions.
CD38 Is Robustly Induced in Human Macrophages and Monocytes in Inflammatory Conditions.
复制标题
DOI：
10.3389/fimmu.2018.01593
复制
发表时间：
2018
影响因子：
7.3
通讯作者：
Guerau-de-Arellano M
中科院分区：
文献类型：
--
作者：
Amici SA;Young NA;Narvaez-Miranda J;Jablonski KA;Arcos J;Rosas L;Papenfuss TL;Torrelles JB;Jarjour WN;Guerau-de-Arellano M
巨噬细胞及其单核细胞前体介导先天免疫反应，并可以促进从促炎到促消退的一系列表型。目前，很少有标记可以对巨噬细胞表型进行稳健的解剖。我们最近在小鼠体外和体内模型中鉴定出 CD38 作为炎症巨噬细胞的标志物。然而，尚不清楚 CD38 在人类巨噬细胞和炎症性疾病中是否发挥类似的标记和/或功能作用。在这里，我们确定 CD38 转录物和蛋白质在暴露于 LPS (±IFN-γ) 炎症刺激的人巨噬细胞中被强烈诱导，但在替代刺激 IL-4 下则不然。药理学和/或遗传性 CD38 功能丧失显着降低了人原代巨噬细胞中炎症细胞因子 IL-6 和 IL-12p40 的分泌以及糖酵解活性。最后，系统性红斑狼疮患者的单核细胞分析表明，虽然所有单核细胞都表达 CD38，但非经典单核细胞亚群中 CD38 的高表达与疾病相关。这些数据与 CD38 在人类巨噬细胞和单核细胞中的炎症标志物作用一致。
Macrophages and their monocyte precursors mediate innate immune responses and can promote a spectrum of phenotypes from pro-inflammatory to pro-resolving. Currently, there are few markers that allow for robust dissection of macrophage phenotype. We recently identified CD38 as a marker of inflammatory macrophages in murine in vitro and in vivo models. However, it is unknown whether CD38 plays a similar marker and/or functional role in human macrophages and inflammatory diseases. Here, we establish that CD38 transcript and protein are robustly induced in human macrophages exposed to LPS (±IFN-γ) inflammatory stimuli, but not with the alternative stimulus, IL-4. Pharmacologic and/or genetic CD38 loss-of-function significantly reduced the secretion of inflammatory cytokines IL-6 and IL-12p40 and glycolytic activity in human primary macrophages. Finally, monocyte analyses in systemic lupus erythematosus patients revealed that, while all monocytes express CD38, high CD38 expression in the non-classical monocyte subpopulation is associated with disease. These data are consistent with an inflammatory marker role for CD38 in human macrophages and monocytes.
登录
查看更多内容
影响因子：
7.3
作者：
Amici SA;Dong J;Guerau-de-Arellano M
通讯作者：
Guerau-de-Arellano M
影响因子：
3.7
作者：
Choe CU;Lardong K;Gelderblom M;Ludewig P;Leypoldt F;Koch-Nolte F;Gerloff C;Magnus T
通讯作者：
Magnus T
DOI：
10.4049/jimmunol.1301758
发表时间：
2014-03-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Gross TJ;Kremens K;Powers LS;Brink B;Knutson T;Domann FE;Philibert RA;Milhem MM;Monick MM
通讯作者：
Monick MM
影响因子：
4.6
作者：
Henriques, Ana;Silva, Isabel;Paiva, Artur
通讯作者：
Paiva, Artur
影响因子：
4.4
作者：
Ancuta P;Liu KY;Misra V;Wacleche VS;Gosselin A;Zhou X;Gabuzda D
通讯作者：
Gabuzda D