PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification.-猫眼课题宝

DOI：

10.1093/nar/gkp1005

发表时间：

2010-01

期刊：

Nucleic acids research

影响因子：

14.9

通讯作者：

中科院分区：

生物学2区

文献类型：

作者：

Spandidos A;Wang X;Wang H;Seed B

来源链接：

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2808898/?report=classic

PrimerBank（http：//pga.mgh.harvard.edu/primerbank/）是用于检索人和小鼠引物对的公共资源，用于通过PCR和定量PCR（QPCR）进行基因表达分析。可以从PrimerBank数据库中访问涵盖大多数已知人类和小鼠基因的总共306 800个引物，以及关于这些引物的信息，例如Tm、转录物上的位置和扩增子大小。对于每个基因，已经设计了至少一个引物对，并且在许多情况下存在替代引物对。引物被设计为在相同的PCR条件下工作，从而促进高通量QPCR。有几种方法来搜索目标基因的引物，例如通过：GenBank登录号、NCBI蛋白质登录号、NCBI基因ID、PrimerBank ID、NCBI基因符号或基因描述（关键字）。总共有26855对引物覆盖了大多数已知的小鼠基因，已经通过QPCR、琼脂糖凝胶分析、测序和BLAST进行了实验验证，所有验证数据都可以从PrimerBank网站免费获得。

PrimerBank (http://pga.mgh.harvard.edu/primerbank/) is a public resource for the retrieval of human and mouse primer pairs for gene expression analysis by PCR and Quantitative PCR (QPCR). A total of 306 800 primers covering most known human and mouse genes can be accessed from the PrimerBank database, together with information on these primers such as Tm, location on the transcript and amplicon size. For each gene, at least one primer pair has been designed and in many cases alternative primer pairs exist. Primers have been designed to work under the same PCR conditions, thus facilitating high-throughput QPCR. There are several ways to search for primers for the gene(s) of interest, such as by: GenBank accession number, NCBI protein accession number, NCBI gene ID, PrimerBank ID, NCBI gene symbol or gene description (keyword). In all, 26 855 primer pairs covering most known mouse genes have been experimentally validated by QPCR, agarose gel analysis, sequencing and BLAST, and all validation data can be freely accessed from the PrimerBank web site.