Transcriptional profiling of Pseudomonas aeruginosa mature single- and dual-species biofilms in response to meropenem.
Transcriptional profiling of Pseudomonas aeruginosa mature single- and dual-species biofilms in response to meropenem.
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DOI：
10.1099/mic.0.001271
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发表时间：
2023-01
期刊：
影响因子：
2.8
通讯作者：
Hall, Rebecca A.
中科院分区：
文献类型：
--
作者：
Alam, Farhana;Blair, Jessica M. A.;Hall, Rebecca A.
铜绿假单胞菌 是一种革兰氏阴性机会致病菌，经常从囊性纤维化肺和烧伤伤口的慢性感染中分离出来，并且是抗微生物剂耐药性医院感染的主要原因。 铜绿假单胞菌 经常与机会性真菌病原体白色念珠菌共分离，存在C.双菌种生物膜中的白色念珠菌促进对美罗培南的耐受性。在这里，成熟的转录谱 铜绿假单胞菌 单种或双种生物膜的研究，以了解C.白念珠菌增强美罗培南耐受性。C.白色念珠菌似乎对转录组有轻微影响， 铜绿假单胞菌 成熟的生物膜，与大多数差异调节基因参与界间的相互作用（即群体感应和吩嗪生物合成）。将美罗培南添加到成熟的单种或双种生物膜中导致显著的细菌转录反应，包括诱导参与生物膜形成的β-内酰胺酶ampC基因。 铜绿假单胞菌 在C. albicans，而C.白念珠菌促进了额外外排泵的表达，这可能在增加耐药性中起作用。 铜绿假单胞菌 美罗培南
Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen frequently isolated from chronic infections of the cystic fibrosis lung and burn wounds, and is a major cause of antimicrobial-resistant nosocomial infections. P. aeruginosa is frequently co-isolated with the opportunistic fungal pathogen Candida albicans, with the presence of C. albicans in dual-species biofilms promoting tolerance to meropenem. Here, transcription profiling of mature P. aeruginosa single- or dual-species biofilms was carried out to understand the molecular mechanism(s) by which C. albicans enhances meropenem tolerance. C. albicans appeared to have a mild impact on the transcriptome of P. aeruginosa mature biofilms, with most differentially regulated genes being involved in interkingdom interactions (i.e. quorum sensing and phenazine biosynthesis). The addition of meropenem to mature single- or dual-species biofilms resulted in a significant bacterial transcriptional response, including the induction of the beta-lactamase, ampC, genes involved in biofilm formation. P. aeruginosa elicited a similar transcriptional response to meropenem in the presence of C. albicans, but C. albicans promoted the expression of additional efflux pumps, which could play roles in increasing the tolerance of P. aeruginosa to meropenem.
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影响因子：
6.4
作者：
Florez C;Raab JE;Cooke AC;Schertzer JW
通讯作者：
Schertzer JW
DOI：
10.3390/antibiotics9090596
发表时间：
2020-09-11
期刊：
Antibiotics (Basel, Switzerland)
影响因子：
--
作者：
Hulen C;Racine PJ;Chevalier S;Feuilloley M;Lomri NE
通讯作者：
Lomri NE
影响因子：
3
作者：
Fuste, Ester;Lopez-Jimenez, Lidia;Vinas, Miguel
通讯作者：
Vinas, Miguel
影响因子：
3.4
作者：
Chen, Lihua;Zou, Yaru;Wu, Yong
通讯作者：
Wu, Yong
影响因子：
3.6
作者：
Dietrich, Lars E. P.;Price-Whelan, Alexa;Newman, Dianne K.
通讯作者：
Newman, Dianne K.