Backbone-free duplex-stacked monomer nucleic acids exhibiting Watson-Crick selectivity.
Backbone-free duplex-stacked monomer nucleic acids exhibiting Watson-Crick selectivity.
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DOI：
10.1073/pnas.1721369115
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发表时间：
2018-08-14
影响因子：
11.1
通讯作者：
Clark NA
中科院分区：
文献类型：
--
作者：
Smith GP;Fraccia TP;Todisco M;Zanchetta G;Zhu C;Hayden E;Bellini T;Clark NA
本文报道的柱状液晶相是最小分子种自组装成核酸双碱基配对堆叠柱状双螺旋结构的物理结合。这些单体的组装可以提供起始状态，能够以高度的选择性将适当的分子从溶液中分离出来，作为分子选择、自组装以及最终的聚合物序列定向组装的益生元出现的途径。我们证明了核酸（NA）单核苷酸三磷酸（dNTPs和rNTPs），在足够高的浓度和低温的水溶液中，可以表现出相变，自发地出现慢性柱状液晶有序。值得注意的是，在NA单体的自组装中，这种无聚合物状态表现出生物核酸的关键结构要素，包括：碱基对的长距离双相堆叠、分子的互补性依赖分配和沃森-克里克选择性，因此，在所有腺苷、胞嘧啶、鸟嘌呤和胸腺嘧啶ntp及其二元混合物的溶液中，双柱状排列在a - t和C-G组合中最稳定。
The columnar liquid crystal phases reported here are physical associations of the smallest molecular species to self-assemble into the duplex base-paired stacked columnar double-helical structures of nucleic acids. These assemblies of monomers can provide starting states capable of partitioning appropriate molecules from solution with a high degree of selectivity, acting as pathways for the prebiotic appearance of molecular selection, self-assembly, and, ultimately, of the sequence-directed assembly of polymers. We demonstrate that nucleic acid (NA) mononucleotide triphosphates (dNTPs and rNTPs), at sufficiently high concentration and low temperature in aqueous solution, can exhibit a phase transition in which chromonic columnar liquid crystal ordering spontaneously appears. Remarkably, this polymer-free state exhibits, in a self-assembly of NA monomers, the key structural elements of biological nucleic acids, including: long-ranged duplex stacking of base pairs, complementarity-dependent partitioning of molecules, and Watson–Crick selectivity, such that, among all solutions of adenosine, cytosine, guanine, and thymine NTPs and their binary mixtures, duplex columnar ordering is most stable in the A-T and C-G combinations.
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影响因子：
5.6
作者：
HOWARD, FB;FRAZIER, J;MILES, HT
通讯作者：
MILES, HT
影响因子：
3.9
作者：
Khanh Thuy Nguyen;Sciortino, Francesco;De Michele, Cristiano
通讯作者：
De Michele, Cristiano
影响因子：
15
作者：
Chami, Fatima;Wilson, Mark R.
通讯作者：
Wilson, Mark R.
DOI：
10.1016/0006-291x(64)90306-7
发表时间：
1964-01-01
影响因子：
3.1
作者：
HOWARD, FB;LIPSETT, MN;FRAZIER, J
通讯作者：
FRAZIER, J
影响因子：
5.5
作者：
De Michele, Cristiano;Bellini, Tommaso;Sciortino, Francesco
通讯作者：
Sciortino, Francesco