Advances in sequencing-based studies of microDNA and ecDNA: Databases, identification methods, and integration with single-cell analysis.
Advances in sequencing-based studies of microDNA and ecDNA: Databases, identification methods, and integration with single-cell analysis.
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DOI：
10.1016/j.csbj.2023.05.017
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发表时间：
2023
影响因子：
6
通讯作者：
Huang, Moli
中科院分区：
文献类型：
--
作者：
Jiang, Rong;Yang, Manqiu;Zhang, Shufan;Huang, Moli
染色体外环状DNA （extrachrosomal circular DNA, eccDNA）是一类起源于基因组DNA但与染色体分离的环状DNA分子。它们在各种生物中很常见，大小从几百到数百万个碱基对不等。一种特殊类型的大染色体外DNA （ecDNA）普遍存在于癌细胞中。对ecDNA的研究极大地促进了我们对癌症发展、进展、进化和耐药性的理解。利用新一代（NGS）和第三代测序（TGS）技术鉴定全基因组eccdna已成为当前研究的一个趋势。在这里，我们简要回顾了两种不同类型的eccdna的生物学机制和应用的最新进展：微dna和ecDNA。除了介绍基于测序数据的现有鉴定工具外，我们还总结了最近将ecDNA与单细胞分析结合起来的努力，并提出了促进这一进程的建议。
Extrachromosomal circular DNA (eccDNA) is a class of circular DNA molecules that originate from genomic DNA but are separate from chromosomes. They are common in various organisms, with sizes ranging from a few hundred to millions of base pairs. A special type of large extrachromosomal DNA (ecDNA) is prevalent in cancer cells. Research on ecDNA has significantly contributed to our comprehension of cancer development, progression, evolution, and drug resistance. The use of next-generation (NGS) and third-generation sequencing (TGS) techniques to identify eccDNAs throughout the genome has become a trend in current research. Here, we briefly review current advances in the biological mechanisms and applications of two distinct types of eccDNAs: microDNA and ecDNA. In addition to presenting available identification tools based on sequencing data, we summarize the most recent efforts to integrate ecDNA with single-cell analysis and put forth suggestions to promote the process.
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