Serum and cerebrospinal fluid tau protein level as biomarkers for evaluating acute spinal cord injury severity and motor function outcome
Serum and cerebrospinal fluid tau protein level as biomarkers for evaluating acute spinal cord injury severity and motor function outcome
复制标题
血清和脑脊液tau蛋白水平作为评估急性脊髓损伤严重程度和运动功能结果的生物标志物
DOI：
10.4103/1673-5374.249238
复制
发表时间：
2019-05
影响因子：
6.1
通讯作者：
Wang Dong Lin
中科院分区：
文献类型：
--
作者：
Tang Ying;Liu Hong Liang;Min Ling Xia;Yuan Hao Shi;Guo Lei;Han Peng Bo;Lu Yu Xin;Zhong Jian Feng;Wang Dong Lin
Tau蛋白是一种微管相关蛋白，在神经元和轴突中有高度特异性表达。由于创伤性脊髓损伤主要影响神经元和轴突，我们推测tau蛋白可能是一个很有前途的反映脊髓损伤程度和运动功能预后的生物标志物。本研究将160只雌性Sprague-Dawley大鼠随机分为假手术组、轻度、中度和重度脊髓损伤组。在所有组中，在T8节段进行椎板切除术以暴露脊髓。使用NYU-MASCIS撞击器，将10 g棒从12.5 mm（轻度）、25 mm（中度）和50 mm（重度）高度跌落至暴露的脊髓背侧表面，造成挫伤性损伤。分别于术后1、6、12、24 h、3、7、14、28 d检测血清和脑脊液中Tau蛋白水平。采用Basso、Beattie和Bresnahan运动评定量表评估所有大鼠的运动功能。三组脊髓损伤后血清和脑脊液中Tau蛋白浓度均迅速升高，并在脊髓损伤后12小时达到峰值。在三个脊髓损伤组中，tau蛋白水平与脊髓损伤严重程度之间以及tau蛋白水平与Basso、Beattie和Bresnahan运动评定量表评分之间存在统计学显著的正线性相关。三个脊髓损伤组中12小时的tau蛋白水平与28天的Basso、Beattie和Bresnahan运动评定量表评分呈负相关（血清：r =-0.94;脑脊液：r =-0.95）。我们的数据表明，血清和脑脊液中的tau蛋白水平可能是预测创伤性脊髓损伤的严重程度和功能结果的一个有希望的生物标志物。
Tau protein, a microtubule-associated protein, has a high specific expression in neurons and axons. Because traumatic spinal cord injury mainly affects neurons and axons, we speculated that tau protein may be a promising biomarker to reflect the degree of spinal cord injury and prognosis of motor function. In this study, 160 female Sprague-Dawley rats were randomly divided into a sham group, and mild, moderate, and severe spinal cord injury groups. A laminectomy was performed at the T8 level to expose the spinal cord in all groups. A contusion lesion was made with the NYU-MASCIS impactor by dropping a 10 g rod from heights of 12.5 mm (mild), 25 mm (moderate) and 50 mm (severe) upon the exposed dorsal surface of the spinal cord. Tau protein levels were measured in serum and cerebrospinal fluid samples at 1, 6, 12, 24 hours, 3, 7, 14 and 28 days after operation. Locomotor function of all rats was assessed using the Basso, Beattie and Bresnahan locomotor rating scale. Tau protein concentration in the three spinal cord injury groups (both in serum and cerebrospinal fluid) rapidly increased and peaked at 12 hours after spinal cord injury. Statistically significant positive linear correlations were found between tau protein level and spinal cord injury severity in the three spinal cord injury groups, and between the tau protein level and Basso, Beattie, and Bresnahan locomotor rating scale scores. The tau protein level at 12 hours in the three spinal cord injury groups was negatively correlated with Basso, Beattie, and Bresnahan locomotor rating scale scores at 28 days (serum: r = −0.94; cerebrospinal fluid: r = −0.95). Our data suggest that tau protein levels in serum and cerebrospinal fluid might be a promising biomarker for predicting the severity and functional outcome of traumatic spinal cord injury.
登录
查看更多内容
DOI：
10.26355/eurrev_201807_15394
发表时间：
2018-07
影响因子：
3.3
作者：
Y. Fan;Min Zhu;D. Geng;K. Zhou;G. Du;Z-L Wang
通讯作者：
Y. Fan;Min Zhu;D. Geng;K. Zhou;G. Du;Z-L Wang
影响因子：
4.2
作者：
M. Segal
通讯作者：
M. Segal
影响因子：
3.2
作者：
M. Barbagallo;G. Vitaliti;F. Greco;P. Pavone;N. Matin;G. Panta;R. Lubrano;R. Falsaperla
通讯作者：
M. Barbagallo;G. Vitaliti;F. Greco;P. Pavone;N. Matin;G. Panta;R. Lubrano;R. Falsaperla
影响因子：
2.8
作者：
Yahata, Kenichiro;Kanno, Haruo;Itoi, Eiji
通讯作者：
Itoi, Eiji
影响因子：
2.2
作者：
Yan X;Zhang A;Yu L;Chen C;Cui H
通讯作者：
Cui H