A WD repeat protein controls the cell cycle and differentiation by negatively regulating Cdc2/B-type cyclin complexes.
A WD repeat protein controls the cell cycle and differentiation by negatively regulating Cdc2/B-type cyclin complexes.
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WD 重复蛋白通过负向调节 Cdc2/B 型细胞周期蛋白复合物来控制细胞周期和分化。
DOI：
10.1091/mbc.8.12.2475
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发表时间：
1997
影响因子：
3.3
通讯作者：
Hiroto Okayama
中科院分区：
文献类型：
--
作者：
Satoko Yamaguchi;H. Murakami;Hiroshi Murakami;Hiroto Okayama
在裂殖酵母裂殖酵母中，p34（cdc 2）在控制细胞周期中起核心作用。我们最近分离到一个新的基因srw 1（+），能够编码一个WD重复蛋白，作为一个多拷贝抑制过度激活的p34（cdc 2）。缺乏srw 1（+）的细胞是不育的，在细胞周期控制中有缺陷。当缺乏氮源时，如果抑制性酪氨酸磷酸化对p34（cdc 2）/Cdc 13的调节被Wee 1激酶的部分失活所破坏，则它们不能有效地停滞在G1期，并死于加速的有丝分裂灾难。通过缺失CD 32 B型细胞周期蛋白或p34（cdc 2）的轻微失活，破坏剂恢复了生育力。srw 1（+）与rum 1（+）具有相似的功能，具有诱导胞内复制和恢复对rum 1破坏物的育性的能力。在srw 1破坏剂，Cdc 13未能被降解时，细胞被氮饥饿。我们的结论是Srw 1控制分化和细胞周期至少通过负调节p34（cdc 2）和Cdc 13相关的p34（cdc 2），它的作用之一是下调有丝分裂细胞周期蛋白的水平，特别是在氮缺乏的环境。
In the fission yeast Schizosaccharomyces pombe, p34(cdc2) plays a central role controlling the cell cycle. We recently isolated a new gene named srw1(+), capable of encoding a WD repeat protein, as a multicopy suppressor of hyperactivated p34(cdc2). Cells lacking srw1(+) are sterile and defective in cell cycle controls. When starved for nitrogen source, they fail to effectively arrest in G1 and die of accelerated mitotic catastrophe if regulation of p34(cdc2)/Cdc13 by inhibitory tyrosine phosphorylation is compromised by partial inactivation of Wee1 kinase. Fertility is restored to the disruptant by deletion of Cig2 B-type cyclin or slight inactivation of p34(cdc2). srw1(+) shares functional similarity with rum1(+), having abilities to induce endoreplication and restore fertility to rum1 disruptants. In the srw1 disruptant, Cdc13 fails to be degraded when cells are starved for nitrogen. We conclude that Srw1 controls differentiation and cell cycling at least by negatively regulating Cig2- and Cdc13-associated p34(cdc2) and that one of its roles is to down-regulate the level of the mitotic cyclin particularly in nitrogen-poor environments.
影响因子：
11.4
作者：
ROWLEY, R;SUBRAMANI, S;YOUNG, PG
通讯作者：
YOUNG, PG