The fate and lifespan of human monocyte subsets in steady state and systemic inflammation.
The fate and lifespan of human monocyte subsets in steady state and systemic inflammation.
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DOI：
10.1084/jem.20170355
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发表时间：
2017-07-03
期刊：
影响因子：
--
通讯作者：
Yona S
中科院分区：
文献类型：
--
作者：
Patel AA;Zhang Y;Fullerton JN;Boelen L;Rongvaux A;Maini AA;Bigley V;Flavell RA;Gilroy DW;Asquith B;Macallan D;Yona S
使用稳定同位素标记，Patel等人建立了在动态平衡中循环的所有三种人单核细胞亚群的寿命;在稳态下，经典单核细胞是短暂的前体细胞，有可能成为中间和非经典单核细胞。他们强调，全身性炎症诱导经典单核细胞紧急释放到循环中。在人类中，单核细胞池包括三个亚群（经典，中间和非经典），在动态平衡中循环。其产生、分化和消失的动力学基础对于理解稳态和炎症反应至关重要。在这里，使用人类在体内氘标记，我们表明，经典的单核细胞出现首先从骨髓中，有丝分裂后的间隔1.6天，并循环一天。随后标记的中间和非经典的单核细胞是一致的顺序过渡的模型。中间型和非经典型单核细胞的循环寿命较长（分别为104和107 d）。在人类实验性内毒素血症模型中，观察到一过性但严重的单核细胞减少症;通过早期从骨髓释放经典单核细胞实现循环单核细胞的恢复。再增殖的顺序概括了健康稳态中的成熟顺序。单核细胞亚群之间的这种发育关系通过将人经典单核细胞移植到人源化小鼠中的命运映射来验证，所述小鼠能够依次分化成中间细胞和非经典细胞。
Using stable isotope labeling, Patel et al. establish the lifespan of all three human monocyte subsets that circulate in dynamic equilibrium; in steady state, classical monocytes are short-lived precursors with the potential to become intermediate and nonclassical monocytes. They highlight that systemic inflammation induces an emergency release of classical monocytes into the circulation. In humans, the monocyte pool comprises three subsets (classical, intermediate, and nonclassical) that circulate in dynamic equilibrium. The kinetics underlying their generation, differentiation, and disappearance are critical to understanding both steady-state homeostasis and inflammatory responses. Here, using human in vivo deuterium labeling, we demonstrate that classical monocytes emerge first from marrow, after a postmitotic interval of 1.6 d, and circulate for a day. Subsequent labeling of intermediate and nonclassical monocytes is consistent with a model of sequential transition. Intermediate and nonclassical monocytes have longer circulating lifespans (∼4 and ∼7 d, respectively). In a human experimental endotoxemia model, a transient but profound monocytopenia was observed; restoration of circulating monocytes was achieved by the early release of classical monocytes from bone marrow. The sequence of repopulation recapitulated the order of maturation in healthy homeostasis. This developmental relationship between monocyte subsets was verified by fate mapping grafted human classical monocytes into humanized mice, which were able to differentiate sequentially into intermediate and nonclassical cells.
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DOI：
10.1084/jem.20120690
发表时间：
2013-11-18
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Avraham-Davidi I;Yona S;Grunewald M;Landsman L;Cochain C;Silvestre JS;Mizrahi H;Faroja M;Strauss-Ayali D;Mack M;Jung S;Keshet E
通讯作者：
Keshet E
影响因子：
82.9
作者：
通讯作者：
--
影响因子：
64.8
作者：
Deng, Kai;Pertea, Mihaela;Rongvaux, Anthony;Wang, Leyao;Durand, Christine M.;Ghiaur, Gabriel;Lai, Jun;McHugh, Holly L.;Hao, Haiping;Zhang, Hao;Margolick, Joseph B.;Gurer, Cagan;Murphy, Andrew J.;Valenzuela, David M.;Yancopoulos, George D.;Deeks, Steven G.;Strowig, Till;Kumar, Priti;Siliciano, Janet D.;Salzberg, Steven L.;Flavell, Richard A.;Shan, Liang;Siliciano, Robert F.
通讯作者：
Siliciano, Robert F.
DOI：
10.3791/53913
发表时间：
2016-05-16
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Fullerton JN;Segre E;De Maeyer RP;Maini AA;Gilroy DW
通讯作者：
Gilroy DW
肺泡巨噬细胞从胎儿单核细胞中发展，这些单核细胞在生命的第一周通过GM-CSF分化为长寿命细胞。
DOI：
10.1084/jem.20131199
发表时间：
2013-09-23
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Guilliams M;De Kleer I;Henri S;Post S;Vanhoutte L;De Prijck S;Deswarte K;Malissen B;Hammad H;Lambrecht BN
通讯作者：
Lambrecht BN