Specification of the patterning of a ductal tree during branching morphogenesis of the submandibular gland.
Specification of the patterning of a ductal tree during branching morphogenesis of the submandibular gland.
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DOI：
10.1038/s41598-020-79650-y
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发表时间：
2021-01-11
影响因子：
4.6
通讯作者：
Kukuruzinska MA
中科院分区：
文献类型：
--
作者：
Walker JL;Wang W;Lin E;Romisher A;Bouchie MP;Bleaken B;Menko AS;Kukuruzinska MA
分支形态发生过程中导管结构的发育依赖于指定导管祖细胞的信号来建立导管网络的模式。在这里，我们将 F-肌动蛋白细胞骨架和细胞粘附蛋白 ZO-1 定义的细胞不对称性确定为胚胎下颌下腺 (SMG) 导管规范的最早决定因素。然后，顶端极性蛋白 aPKC z 以 ZO-1 依赖性方式被招募到不对称位点，并与 ROCK 信号传导协作，建立导管祖细胞的顶端-基底极性，并进一步定义导管规范的路径。此外，运动蛋白肌球蛋白IIB是沿着肌动蛋白丝传递机械力的介质，在导管腔形成过程中定位于连接多个导管上皮细胞顶端域的顶点，并驱动导管成熟。这些研究确定细胞骨架蛋白、连接蛋白和极性蛋白是 SMG 分支形态发生过程中导管规格和导管树模式的早期决定因素。
The development of ductal structures during branching morphogenesis relies on signals that specify ductal progenitors to set up a pattern for the ductal network. Here, we identify cellular asymmetries defined by the F-actin cytoskeleton and the cell adhesion protein ZO-1 as the earliest determinants of duct specification in the embryonic submandibular gland (SMG). Apical polarity protein aPKCζ is then recruited to the sites of asymmetry in a ZO-1-dependent manner and collaborates with ROCK signaling to set up apical-basal polarity of ductal progenitors and further define the path of duct specification. Moreover, the motor protein myosin IIB, a mediator of mechanical force transmission along actin filaments, becomes localized to vertices linking the apical domains of multiple ductal epithelial cells during the formation of ductal lumens and drives duct maturation. These studies identify cytoskeletal, junctional and polarity proteins as the early determinants of duct specification and the patterning of a ductal tree during branching morphogenesis of the SMG.
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影响因子：
2.7
作者：
Daley, William P.;Gulfo, Kathryn M.;Sequeira, Sharon J.;Larsen, Melinda
通讯作者：
Larsen, Melinda
影响因子：
4.4
作者：
Gluck C;Min S;Oyelakin A;Smalley K;Sinha S;Romano RA
通讯作者：
Romano RA
影响因子：
11.8
作者：
Hayashi T;Lombaert IM;Hauser BR;Patel VN;Hoffman MP
通讯作者：
Hoffman MP
影响因子：
4.6
作者：
Hoffman, MP;Kidder, BL;Larsen, M
通讯作者：
Larsen, M
影响因子：
2.7
作者：
Larsen, M;Hoffman, MP;Yamada, KM
通讯作者：
Yamada, KM