Emergence of community-associated methicillin-resistant Staphylococcus aureus associated with pediatric infection in Cambodia.
Emergence of community-associated methicillin-resistant Staphylococcus aureus associated with pediatric infection in Cambodia.
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DOI：
10.1371/journal.pone.0006630
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发表时间：
2009-08-13
期刊：
影响因子：
3.7
通讯作者：
Peacock SJ
中科院分区：
文献类型：
--
作者：
Chheng K;Tarquinio S;Wuthiekanun V;Sin L;Thaipadungpanit J;Amornchai P;Chanpheaktra N;Tumapa S;Putchhat H;Day NP;Peacock SJ
在发达国家，社区相关的耐甲氧西林金黄色葡萄球菌 (CA-MRSA) 感染的发病率正在上升，但在发展中国家似乎很少见。对这种差异的一种解释是，资源匮乏的国家缺乏检测 CA-MRSA 携带和感染所必需的诊断微生物学设施。 2006 年 1 月，我们在柬埔寨西部暹粒的吴哥儿童医院开发了诊断微生物学能力，同月发现一名儿童患有由 CA-MRSA 引起的严重社区获得性脓疱疮。进行了一项研究来识别和描述 2006 年 1 月至 2007 年 12 月期间出现的其他病例。使用多位点序列分型、葡萄球菌盒式染色体 mec (SCCmec) 分型以及 PCR 来确定编码 Panton-Valentine Leukocidin (PVL) 的基因是否存在，对细菌分离株进行了分子表征。 17 名儿童被确诊为 CA-MRSA 感染，其中 11 名儿童患有皮肤和软组织感染，6 名儿童患有侵袭性疾病。大多数案件在时间或地点上无关。分子表征鉴定出两个独立的 MRSA 克隆； 15 个分离株为序列类型 (ST) 834、SCCmec IV 型、PVL 基因阴性，2 个分离株为 ST 121、SCCmec V 型、PVL 基因阳性。这是柬埔寨首次报告耐甲氧西林金黄色葡萄球菌（MRSA），其传播将对公众健康构成重大威胁。病例大多在时间或地点上无关的发现表明，这些病例是 CA-MRSA 携带者或携带者接触者中的散发性感染，而不是在疫情爆发的情况下发生的。
The incidence of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infection is rising in the developed world but appears to be rare in developing countries. One explanation for this difference is that resource poor countries lack the diagnostic microbiology facilities necessary to detect the presence of CA-MRSA carriage and infection. We developed diagnostic microbiology capabilities at the Angkor Hospital for Children, Siem Reap, western Cambodia in January 2006 and in the same month identified a child with severe community-acquired impetigo caused by CA-MRSA. A study was undertaken to identify and describe additional cases presenting between January 2006 and December 2007. Bacterial isolates underwent molecular characterization using multilocus sequence typing, staphylococcal cassette chromosome mec (SCCmec) typing, and PCR for the presence of the genes encoding Panton-Valentine Leukocidin (PVL). Seventeen children were identified with CA-MRSA infection, of which 11 had skin and soft tissue infection and 6 had invasive disease. The majority of cases were unrelated in time or place. Molecular characterization identified two independent MRSA clones; fifteen isolates were sequence type (ST) 834, SCCmec type IV, PVL gene-negative, and two isolates were ST 121, SCCmec type V, PVL gene-positive. This represents the first ever report of MRSA in Cambodia, spread of which would pose a significant threat to public health. The finding that cases were mostly unrelated in time or place suggests that these were sporadic infections in persons who were CA-MRSA carriers or contacts of carriers, rather than arising in the context of an outbreak.
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影响因子：
9.4
作者：
Gupta, Kalpana;MacIntyre, Ann;Dembry, Louise-Marie
通讯作者：
Dembry, Louise-Marie
DOI：
10.1016/j.diagmicrobio.2008.07.016
发表时间：
2008-12-01
影响因子：
2.9
作者：
Sam, I-Ching;Kahar-Bador, Maria;Ghazali, Fadzilah Mohd Nor
通讯作者：
Ghazali, Fadzilah Mohd Nor
影响因子：
9.4
作者：
Okuma, K;Iwakawa, K;Hiramatsu, K
通讯作者：
Hiramatsu, K
影响因子：
--
作者：
Hersh, Adam L.;Chambers, Henry F.;Gonzales, Ralph
通讯作者：
Gonzales, Ralph
影响因子：
5.2
作者：
Adhikari, RP;Cook, GM;Smith, JMB
通讯作者：
Smith, JMB