Restraint stress enhances the gene expression of prolactin receptor long form at the choroid plexus.
Restraint stress enhances the gene expression of prolactin receptor long form at the choroid plexus.
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束缚应激增强了脉络丛催乳素受体长型的基因表达。
DOI：
10.1210/endo.136.12.7588315
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发表时间：
1995
期刊：
影响因子：
4.8
通讯作者：
Kunio Nakashima
中科院分区：
文献类型：
--
作者：
T. Fujikawa;Hideaki Soya;H. Yoshizato;K. Sakaguchi;Katsumi Doh;Minoru Tanaka;Kunio Nakashima
大脑功能的激素控制被认为是重要的压力的耐受性，它是现在建立的压力升高血清PRL水平在雄性或循环雌性大鼠。为了研究应激过程中血清PRL是否或如何作用于大脑，我们分析了水中束缚应激（RSW）大鼠的血清PRL水平和大脑PRL受体的基因表达。RSW暴露30 min内大鼠血清PRL浓度显著升高，4 h后下降至初始水平，并在此水平上维持7 h。应激解除后，大鼠血清催乳素水平在6 h内明显降低。核糖核酸酶保护试验和原位杂交分析表明，RSW 2 h可诱导大鼠脉络丛长型PRL受体[PRL-R（L）] mRNA表达。应激解除后，大鼠海马PRL-R（L）mRNA的高表达水平降低。RSW治疗前和治疗过程中，下丘脑PRL-R（L）mRNA表达水平低于脉络丛。在RSW前或RSW过程中，大鼠脑内短型PRL受体mRNA的表达明显低于长型受体mRNA的表达。结果表明，束缚应激引起血清PRL水平迅速升高，并诱导脉络丛PRL-R（L）基因表达，提示应激诱导和脉络丛PRL-R（L）介导血清PRL向脑脊液的转运。
Hormonal control of brain functions is considered to be important in the tolerance of stress, and it is now established that stress elevates serum PRL levels in male or cycling female rats. To investigate whether or how serum PRL acts on the brain during exposure to stress, we analyzed serum PRL levels and the gene expression of brain PRL receptors in rats subjected to restraint stress in the water (RSW). The serum PRL concentration was remarkably increased within 30 min in the rats by exposure to RSW and decreased to the initial level after 4 h of RSW, remaining at this level for up to 7 h of RSW. After the rats were released from the stress, the serum PRL level was significantly lowered in 6 h. Ribonuclease protection assay and in situ hybridization analysis revealed that messenger RNA (mRNA) expression for the long form PRL receptor [PRL-R(L)] was remarkably induced in the rat choroid plexus in 2 h of RSW. The high expression level of PRL-R(L) mRNA in the region was reduced after the rats were released from the stress. PRL-R(L) mRNA expression in the hypothalamus was at lower levels than those in the choroid plexus before and during the RSW treatment. The short form PRL receptor mRNA expression in the rat brain was considerably lower than expression of the long form receptor mRNA before or during RSW. The results indicated that the restraint stress caused a rapid increase in serum PRL and induced the gene expression for PRL-R(L) in the choroid plexus, suggesting stress-induced and choroid plexus PRL-R(L)-mediated transport of serum PRL into the cerebrospinal fluid.
影响因子：
4.8
作者：
S. Chiu;R. D. Koos;P. M. Wise
通讯作者：
S. Chiu;R. D. Koos;P. M. Wise
将催乳素从血液转运至脑脊液的受体介导机制。
影响因子：
4.8
作者：
Walsh,RJ;Slaby,FJ;Posner,BI
通讯作者：
Posner,BI
束缚应激会降低年轻雌性大鼠结节漏斗部多巴胺能神经元的神经分泌活性，但不会降低老年雌性大鼠的神经分泌活性。
影响因子：
4.1
作者：
Demarest,KT;Moore,KE;Riegle,GD
通讯作者：
Riegle,GD