Ciguatera toxins in wild coral reef fish along the southern coast of China
Ciguatera toxins in wild coral reef fish along the southern coast of China
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中国南部沿海野生珊瑚礁鱼类中的雪卡毒素
DOI：
10.1071/mf14215
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发表时间：
2015-12
影响因子：
1.8
通讯作者：
Tian-jiu Jiang
中科院分区：
文献类型：
--
作者：
Ni Wu;Qingliu Huan;Kemei Du;Rong Hu;Tian-jiu Jiang
分析了我国南部沿海野生珊瑚鱼体内的雪卡毒素。Cigua-Check试剂盒用于初步筛选。使用小鼠生物测定来定量通过Cigua-Check试剂盒鉴定为阳性的样品的毒素。采用高效液相色谱-串联质谱法（HPLC-MS/MS）进一步分析小鼠生物测定法鉴定为阳性的样品中的雪卡毒素P-CTX-1。小鼠生物测定结果表明，所有6个采样点都存在雪卡毒鱼类，47.8%的样品被确认受到CTX污染。涉及的物种主要属于毛齿鱼科、鲹科、全齿鱼科、笛鲷科和鲤科。经小白鼠生物测定，P-CTX对鱼肉的毒性范围为0 ~ 169 ng/kg。鱼毒性与鱼的体重、体长和食性无显著相关性。Cigua-Check试剂盒经小鼠生物测定，判断毒、无毒的准确率达95.7%。然而，Cigua-Check试剂盒和小鼠生物测定在毒性测量方面存在显着差异。根据HPLC-MS/MS，在小鼠生物测定鉴定为阳性的任何样品中均未鉴定出P-CTX-1。
Ciguatera toxins (CTXs) in wild coral reef fish collected from the southern coast of China were analysed. Cigua-Check kits were used for primary screening. Mouse bioassay was used to quantify toxins of the samples identified as positive by the Cigua-Check kit. High-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) was used to further analyse P-CTX-1 of ciguatera toxins in the samples identified as positive by the mouse bioassay. The mouse bioassay showed that ciguatoxic fish existed in all six sampling sites, and 47.8% of the samples were confirmed to be contaminated with CTXs. The implicated species largely belonged to Chaetodontidae, Scaridae, Holocentridae, Lutjanidae and Serranidae. The toxicities varied from 0 to 169ng P-CTX-1kg–1 fish flesh according to mouse bioassay. There was no significant correlation between fish toxicity and fish weight, length and feeding habits. The accuracy rate of the Cigua-Check kit in judging toxicity or non-toxicity was up to 95.7% according to the mouse bioassay. However, the Cigua-Check kit and mouse bioassay differed significantly with respect to measures of toxicity. According to HPLC–MS/MS, no P-CTX-1 was identified in any of the samples that the mouse bioassay identified as positive.
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影响因子：
2.8
作者：
Oshiro, Naomasa;Yogi, Kentaro;Inafuku, Yasuo
通讯作者：
Inafuku, Yasuo
影响因子：
6.6
作者：
M. Parsons;L. Preskitt
通讯作者：
M. Parsons;L. Preskitt
DOI：
10.1046/j.1526-0992.1999.09903.x
发表时间：
1999-03-01
期刊：
ECOSYSTEM HEALTH
影响因子：
--
作者：
Hales, S;Weinstein, P;Woodward, A
通讯作者：
Woodward, A
影响因子：
2.8
作者：
Lewis, RJ
通讯作者：
Lewis, RJ
DOI：
10.2307/1442105
发表时间：
1969-06
期刊：
--
影响因子：
--
作者：
Robert S. Jones;Bruce W. Halstead
通讯作者：
Robert S. Jones;Bruce W. Halstead