Detection of c‐erbB‐2 (HER‐2/neu) amplification in breast carcinoma by fluorescence in situ hybridization on tissue sections and imprinted cells
Detection of c‐erbB‐2 (HER‐2/neu) amplification in breast carcinoma by fluorescence in situ hybridization on tissue sections and imprinted cells
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通过组织切片和印迹细胞荧光原位杂交检测乳腺癌中的 c-erbB-2 (HER-2/neu) 扩增
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
A. Ooi
中科院分区：
文献类型：
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作者：
K. Kunitomo;Takuo Takehana;S. Inoue;H. Fujii;Shioto Suzuki;Y. Matsumoto;A. Ooi
c-erbB-2 的异常引起了广泛的关注。使用针对 c-erbB-2 基因产物的抗体进行治疗可有效对抗 c-erbB-2 扩增和/或过度表达的乳腺癌。迫切需要建立方法来选择将从这种疗法中受益的患者。通过免疫组织化学检查了总共 235 例乳腺癌的 c-erbB-2 蛋白过度表达。使用 c-erbB-2 和 17 号染色体着丝粒区域特异的探针，通过双色荧光原位杂交 (FISH) 检查 52 个肿瘤、70 个阴性肿瘤和 35 名患者涂片印记的可辨别免疫染色的组织切片。基因扩增和蛋白质过表达之间的一致性为 95.7%。当比较两种 FISH 制备技术的结果时，发现 24 个肿瘤中没有发现差异。然而，在八个案例中发现了差异。在其中 6 个案例中，差异并不影响扩增的存在或不存在，但在另外两个案例中，被认为在石蜡切片上显示出低水平扩增，而是检测到了多体性 17。结论是，FISH 是检测基因扩增的绝佳工具，尤其是接触印记上的 FISH 是区分低水平扩增和多体性 17 的有用辅助手段。
Abnormalities in c‐erbB‐2 have attracted a great deal of attention. Treatment using an antibody against the c‐erbB‐2 gene product is effective against breast cancers with amplification and/or overexpression of c‐erbB‐2. There is an urgent need to establish methodology for selecting patients who would benefit from this therapy. A total of 235 breast carcinomas were examined for c‐erbB‐2 protein overexpression by immunohistochemistry. Tissue sections with discernible immunostaining from 52 tumors, 70 negative tumors and smear imprints from 35 patients were examined by dual‐color fluorescence in situ hybridization (FISH) using probes specific for c‐erbB‐2 and the centromeric region of chromosome 17. The concordance between gene amplification and protein overexpression was 95.7%. When the findings of the two FISH preparation techniques were compared, no discrepancies were found in 24 of the tumors. However, differences were seen in eight cases. In six of these cases the differences did not affect the presence or absence of amplification, but in the other two cases, considered to show low‐level amplification on paraffin sections, polysomy 17 was detected instead. It was concluded that FISH is an excellent tool to detect gene amplification in particular, and FISH on touch imprints is a useful adjunct to differentiate between low‐level amplification and polysomy 17.
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影响因子：
56.9
作者：
SLAMON, DJ;CLARK, GM;MCGUIRE, WL
通讯作者：
MCGUIRE, WL
荧光原位杂交用于检测乳腺肿瘤细针抽吸活检中的 erbB-2 扩增。
影响因子：
1.8
作者：
Sauter,G;Feichter,G;Torhorst,J;Moch,H;Novotna,H;Wagner,U;Dürmüller,U;Waldman,FM
通讯作者：
Waldman,FM
影响因子：
45.3
作者：
Press, MF;Bernstein, L;Flom, KJ
通讯作者：
Flom, KJ
档案组织样本中 HER-2/neu 抗体的敏感性：癌基因表达免疫组织化学研究中潜在的错误来源。
影响因子：
11.2
作者：
Press,MF;Hung,G;Godolphin,W;Slamon,DJ
通讯作者：
Slamon,DJ