The careful control of Polo kinase by APC/C-Ube2C ensures the intercellular transport of germline centrosomes during Drosophila oogenesis.
The careful control of Polo kinase by APC/C-Ube2C ensures the intercellular transport of germline centrosomes during Drosophila oogenesis.
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APC/C-Ube2C对Polo激酶的精心控制确保了果蝇卵发生过程中种系中心体的细胞间运输。
DOI：
10.1098/rsob.200371
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发表时间：
2021-06
期刊：
影响因子：
5.8
通讯作者：
Kimata Y
中科院分区：
文献类型：
--
作者：
Braun AL;Meghini F;Villa-Fombuena G;Guermont M;Fernandez-Martinez E;Qian Z;Dolores Martín-Bermudo M;González-Reyes A;Glover DM;Kimata Y
后生动物繁殖的一个特征是从卵母细胞中消除母体中心体。在卵子发生过程中形成合体包囊的动物，包括果蝇和人类，包囊内的所有中心体都迁移到卵母细胞，在那里它们随后退化。这一事件的重要性和潜在机制仍不清楚。在这里，我们表明，在果蝇早期卵子发生过程中，控制后期促进复合体/环体(APC/C)，这是细胞周期控制所必需的泛素连接酶复合体，通过调节Polo/Plk1激酶，确保中心体正确地运输到卵母细胞中，Polo/Plk1激酶是中心体完整性和活性的关键调节因子。我们发现，APC/C特异性E2，Vihar/Ube2c的新突变，影响其对APC/C的抑制调节，导致早熟Polo降解，并通过中心体的不稳定阻碍中心体运输。中心体迁移的失败与囊内微管极化的减弱有关，并允许哺育细胞中的异位微管成核，导致卵母细胞特性的丧失。这些结果表明，中心体迁移通过浓缩和限制微管成核活动进入卵母细胞而在维持卵母细胞命运中发挥作用。考虑到APC/C和Polo在整个动物界中的保守作用，我们的发现可能会被翻译到其他动物身上。
A feature of metazoan reproduction is the elimination of maternal centrosomes from the oocyte. In animals that form syncytial cysts during oogenesis, including Drosophila and human, all centrosomes within the cyst migrate to the oocyte where they are subsequently degenerated. The importance and the underlying mechanism of this event remain unclear. Here, we show that, during early Drosophila oogenesis, control of the Anaphase Promoting Complex/Cyclosome (APC/C), the ubiquitin ligase complex essential for cell cycle control, ensures proper transport of centrosomes into the oocyte through the regulation of Polo/Plk1 kinase, a critical regulator of the integrity and activity of the centrosome. We show that novel mutations in the APC/C-specific E2, Vihar/Ube2c, that affect its inhibitory regulation on APC/C cause precocious Polo degradation and impedes centrosome transport, through destabilization of centrosomes. The failure of centrosome migration correlates with weakened microtubule polarization in the cyst and allows ectopic microtubule nucleation in nurse cells, leading to the loss of oocyte identity. These results suggest a role for centrosome migration in oocyte fate maintenance through the concentration and confinement of microtubule nucleation activity into the oocyte. Considering the conserved roles of APC/C and Polo throughout the animal kingdom, our findings may be translated into other animals.
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影响因子：
56.9
作者：
Lei, Lei;Spradling, Allan C.
通讯作者：
Spradling, Allan C.
DOI：
10.1007/978-1-4939-2851-4_7
发表时间：
2015-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Legent, Kevin;Tissot, Nicolas;Guichet, Antoine
通讯作者：
Guichet, Antoine
影响因子：
1.6
作者：
CARPENTER, ATC
通讯作者：
CARPENTER, ATC
细胞周期的时间重塑伴随果蝇种系分化。
影响因子：
2.7
作者：
Hinnant TD;Alvarez AA;Ables ET
通讯作者：
Ables ET
影响因子：
9.2
作者：
Arquint, Christian;Nigg, Erich A.
通讯作者：
Nigg, Erich A.