Effect of dough mixing on wheat endosperm cell walls.
Effect of dough mixing on wheat endosperm cell walls.
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面团混合对小麦胚乳细胞壁的影响。
DOI：
10.1021/jf304677u
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发表时间：
2013
影响因子：
6.1
通讯作者：
Toole GA
中科院分区：
文献类型：
--
作者：
Toole GA
荧光显微镜观察表明，超速离心法分离的面团细胞壁碎片主要来源于淀粉胚乳，部分来自糊粉层和外层。傅立叶变换红外光谱(FTIR)和核磁共振氢谱(1H核磁共振)分析表明，与薄粒切片相比，面团搅拌对细胞壁碎片的结构和组成影响不大。这些分析证实，碎片主要由水不可提取的阿拉伯木聚糖和β-葡聚糖组成。从5个面包小麦品种的面团细胞壁制得的FTIR显微光谱显示，其中两个主要由高度取代的阿拉伯木聚糖(cv.马尼特和圣帕斯托雷)，一种由低、中和高取代阿拉伯木聚糖的混合物组成(cv.Hereward)，其余两个含有较大比例的低取代阿拉伯木聚糖(cv.克莱尔和玉麦(34岁)。豫麦34的细胞壁物质含量较高，其细胞壁中含有较高比例的阿拉伯木聚糖。这种方法将允许在未来调查烘焙原料和加工对胚乳细胞的影响，包括添加木聚糖酶，以确保小麦育种产生的任何潜在健康益处在到达消费者的食品中实现。
Dough-derived cell wall fragments isolated by ultracentrifugation were largely derived from the starchy endosperm, with some fragments deriving from the aleurone and outer layers, as indicated by fluorescence microscopy. Dough mixing had little effect on the structure and composition of cell wall fragments compared to thin grain sections, as determined by Fourier transform infrared (FTIR) and1H nuclear magnetic resonance (NMR) spectroscopy. These analyses confirmed that the fragments largely comprised water-unextractable arabinoxylan and β-glucan. FTIR microspectroscopy of dough-derived cell wall fragments prepared from five bread wheat cultivars showed that two largely comprised highly substituted arabinoxylan (cv. Manital and San Pastore), one comprised a mixture of low, medium, and highly substituted arabinoxylan (cv. Hereward), and the remaining two comprised a greater proportion of low substituted arabinoxylan (cv. Claire and Yumai 34). Yumai 34 yielded a greater mass of cell wall material, and its cell walls comprised a high proportion of medium substituted arabinoxylan. Such methods will allow for the impact of bakery ingredients and processing on endosperm cells, including the addition of xylanases, to be investigated in the future to ensure any potential health benefits arising from wheat breeding are realized in the food that reaches the consumer.
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DOI：
--
发表时间：
1982
期刊：
影响因子：
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作者：
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通讯作者：
R. Fulcher
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DOI：
--
发表时间：
1986
期刊：
影响因子：
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作者：
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影响因子：
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作者：
J. Robertson;L. Sutcliffe;E. Mills
通讯作者：
E. Mills
DOI：
10.1071/bi9730793
发表时间：
1973-01-01
期刊：
AUSTRALIAN JOURNAL OF BIOLOGICAL SCIENCES
影响因子：
--
作者：
MARES, DJ;STONE, BA
通讯作者：
STONE, BA
影响因子：
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作者：
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通讯作者：
Courtin, Christophe M.