Analyses of mixed melanogenesis in tyrosinase cDNA-transfected human amelanotic melanoma cells.
Analyses of mixed melanogenesis in tyrosinase cDNA-transfected human amelanotic melanoma cells.
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酪氨酸酶 cDNA 转染的人无黑色素瘤细胞中混合黑色素生成的分析。
DOI：
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发表时间：
1993
影响因子：
6.5
通讯作者：
M. Kurimoto
中科院分区：
文献类型：
--
作者：
O. Ando;Y. Mishima;S. Hanada;Y. Suemoto;J. Atobe;M. Kurimoto
在色素细胞中，酪氨酸酶的合成和前黑素体的形成是独立的，但协调的过程。然而，这两个过程之间的相互关系以前没有阐明。在这项研究中，人酪氨酸酶cDNA的表达质粒的构建和转染到人无黑色素性黑色素瘤细胞系，G-361。获得稳定的转染细胞（G-CMHT-3），具有高酪氨酸酶活性，并发生明显的黑化。至于黑色素的类型，在G-CMHT-3细胞中，嗜铬黑素和真黑素含量均增加。有趣的是，过氧化氢酶活性作为其他黑素生成酶之一，在G-CMHT-3细胞中降低。过氧化氢酶活性的降低被认为在黑色素聚合物的形成中起作用，导致嗜黑素和真黑素含量的增加。电镜下可见染色的G-CMHT-3细胞中存在多巴氧化酶阳性的高尔基体相关的内质网、溶酶体、包被囊泡和前黑素体，表达的酪氨酸酶被很好地转运到这些细胞器中。此外，与G-361细胞相比，G-CMHT-3细胞中的前黑素体（I-III期）和黑素体（IV期）的数量增加。还注意到，G-CMHT-3细胞显示出更多的正常表型前黑素体，偶尔过渡性前黑素体在其同心螺旋状内膜内显示出部分黑素聚合物形成。G-CMHT-3细胞中真黑素体的数量明显多于G-361细胞。这些结果表明，酪氨酸酶通过其cDNA转染引入诱导活性和结构非异常的前黑素体形成，导致上调的脱黑素和真黑素生成。
In the pigment cell the synthesis of tyrosinase and the formation of premelanosomes are independent, yet coordinated, processes. However, the interrelationship between the two processes has not been elucidated previously. In this study, an expression plasmid for human tyrosinase cDNA was constructed and transfected into a human amelanotic melanoma cell line, G-361. Stable transfected cells (G-CMHT-3) were obtained with high tyrosinase activity and distinct melanization occurred. As for the type of melanin, both pheo- and eumelanin contents increased in G-CMHT-3 cells. Interestingly, catalase activity as one of the other melanogenic enzymes was decreased in G-CMHT-3 cells. The decrease of catalase activity was considered to play a role in melanin-polymer formation, resulting in the increase of both pheo- and eumelanin contents. Under electron microscopic observation, dopa-oxidase-positive Golgi-associated endoplasmic reticulum of lysosome, coated vesicles, and premelanosomes were observed in pigmented G-CMHT-3 cells, and the expressed tyrosinase was considered to be well translocated to these organelles. In addition, the number of premelanosomes (stages I-III) as well as melanosomes (stage IV) increased in G-CMHT-3 cells compared to those in G-361 cells. It is also noted that G-CMHT-3 cells showed more normal phenotype premelanosomes with occasional transitional premelanosomes exhibiting partial melanin polymer formation within their concentric whorl-like internal membranes. Furthermore, the number of eumelanosomes in G-CMHT-3 cells was much larger than that in G-361 cells. These results suggest that the tyrosinase introduced by its cDNA transfection induced active and structurally non-aberrant premelanosome formation resulting in the upregulated pheo- and eumelanogenesis.
外源性 MSH 对 C57BL/6J-Ay/a 毛球黑素细胞内从褐变到真黑素生成的影响。
DOI：
10.1111/1523-1747.ep12515889
发表时间：
1991
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Granholm,NH;VanAmerongen,AW
通讯作者：
VanAmerongen,AW
DOI：
10.1073/pnas.87.12.4809
发表时间：
1990-06-01
影响因子：
11.1
作者：
HALABAN, R;MOELLMANN, G
通讯作者：
MOELLMANN, G