High-density lipoproteins mediate small RNA intercellular communication between dendritic cells and macrophages.
High-density lipoproteins mediate small RNA intercellular communication between dendritic cells and macrophages.
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DOI：
10.1016/j.jlr.2023.100328
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发表时间：
2023-03
影响因子：
6.5
通讯作者：
Vickers, Kasey C.
中科院分区：
文献类型：
--
作者：
Castleberry, Mark;Raby, Chase A.;Ifrim, Anca;Shibata, Yasuhiro;Matsushita, Sachi;Ugawa, Shinya;Miura, Yutaka;Hori, Atsushi;Miida, Takashi;Linton, MacRae F.;Michell, Danielle L.;Tsujita, Maki;Vickers, Kasey C.
HDL 是多种分子货物的动态转运蛋白，在脂质代谢和炎症中发挥着关键作用。我们之前曾报道过，基于定量 PCR 和 sRNA 测序方法，HDL 可以转运宿主和非宿主小 RNA (sRNA)；然而，这些方法需要 RNA 分离步骤，这些步骤存在潜在偏差，并且可能无法从样品中分离出某些形式的 RNA 分子。据报道，HDL 还可以接受来自供体巨噬细胞的功能性 sRNA，并将其递送至受体内皮细胞；然而，使用 PCR 追踪 HDL-sRNA 细胞间通讯有很大的局限性。本研究旨在克服这些技术障碍，并进一步了解 HDL 介导的免疫细胞之间 sRNA 双向流动所涉及的途径。为了克服这些技术限制，使用脂质渗透性 RNA 染料 SYTO RNASelect 来量化 a) HDL-sRNA 总体含量，b) HDL 和免疫细胞之间 sRNA 的双向通量，c) HDL 介导的免疫细胞之间的细胞间通讯，以及 d) HDL 介导的疾病中的 RNA 输出变化。活细胞成像和功能丧失分析表明，内溶酶体系统在巨噬细胞储存和 HDL-sRNA 输出中发挥着关键作用。这些结果确定 HDL 是免疫细胞之间细胞间通讯的实质性介质，并证明内吞作用对于 HDL-sRNA 受体细胞的重要性。利用脂质穿透性 RNA 特异性荧光染料，我们能够量化 HDL 转运的 sRNA 的绝对浓度，并表征 HDL 介导的免疫细胞之间的细胞间 RNA 转运。
HDL are dynamic transporters of diverse molecular cargo and play critical roles in lipid metabolism and inflammation. We have previously reported that HDL transport both host and nonhost small RNAs (sRNA) based on quantitative PCR and sRNA sequencing approaches; however, these methods require RNA isolation steps which have potential biases and may not isolate certain forms of RNA molecules from samples. HDL have also been reported to accept functional sRNAs from donor macrophages and deliver them to recipient endothelial cells; however, using PCR to trace HDL-sRNA intercellular communication has major limitations. The present study aims to overcome these technical barriers and further understand the pathways involved in HDL-mediated bidirectional flux of sRNAs between immune cells. To overcome these technical limitations, SYTO RNASelect, a lipid-penetrating RNA dye, was used to quantify a) overall HDL-sRNA content, b) bidirectional flux of sRNAs between HDL and immune cells, c) HDL-mediated intercellular communication between immune cells, and d) HDL-mediated RNA export changes in disease. Live cell imaging and loss-of-function assays indicate that the endo-lysosomal system plays a critical role in macrophage storage and export of HDL-sRNAs. These results identify HDL as a substantive mediator of intercellular communication between immune cells and demonstrate the importance of endocytosis for recipient cells of HDL-sRNAs. Utilizing a lipid-penetrating RNA-specific fluorescence dye, we were able to both quantify the absolute concentration of sRNAs transported by HDL and characterize HDL-mediated intercellular RNA transport between immune cells.
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影响因子：
5.8
作者：
Vickers KC;Michell DL
通讯作者：
Michell DL
影响因子：
6.5
作者：
Whitacre BE;Howles P;Street S;Morris J;Swertfeger D;Davidson WS
通讯作者：
Davidson WS
影响因子：
4.6
作者：
Brennan, K.;Martin, K.;Mc Gee, M. M.
通讯作者：
Mc Gee, M. M.
影响因子：
4.2
作者：
Olson, RE
通讯作者：
Olson, RE
DOI：
10.1161/atvbaha.116.307028
发表时间：
2016-06
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Canfrán-Duque A;Lin CS;Goedeke L;Suárez Y;Fernández-Hernando C
通讯作者：
Fernández-Hernando C