Boundary cap neural crest stem cells homotopically implanted to the injured dorsal root transitional zone give rise to different types of neurons and glia in adult rodents.
Boundary cap neural crest stem cells homotopically implanted to the injured dorsal root transitional zone give rise to different types of neurons and glia in adult rodents.
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DOI：
10.1186/1471-2202-15-60
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发表时间：
2014-05-05
期刊：
影响因子：
2.4
通讯作者：
Kozlova EN
中科院分区：
文献类型：
--
作者：
Trolle C;Konig N;Abrahamsson N;Vasylovska S;Kozlova EN
边界帽是一组短暂的神经嵴衍生细胞，位于发育过程中感觉轴突进入脊髓时假定的背根过渡区（DRTZ）。随后，这些细胞迁移至背根神经节并分化为感觉神经元和神经胶质细胞的亚型。出生后，当 DRTZ 建立时，感觉轴突不再能够进入脊髓。在这里，我们探讨了背根撕脱后植入受损 DRTZ 的小鼠边界帽神经嵴干细胞 (bNCSC) 的命运，以了解它们协助感觉轴突再生的潜力。移植细胞在移植到撕脱的 DRTZ 后表现出广泛的存活和分化。位于脊髓外部的移植细胞组织了Sox2/GFAP表达细胞的细长管，与再生感觉轴突密切相关，或者在脊髓表面以小簇形式出现。其他细胞作为单细胞迁移到宿主脊髓中，分化为具有不同神经递质特征、广泛纤维组织的脊髓神经元，并且在某些情况下被谷氨酸末端样轮廓包围。这些发现表明，植入背根撕脱损伤部位的 bNCSC 在脊髓内部和外周室中表现出显着的分化可塑性，在外周室中它们组织与再生感觉纤维相关的管。这些特性为探索 bNCSC 协助感觉轴突再生到脊髓并替代受损脊髓中丢失的神经元的能力提供了基础。
The boundary cap is a transient group of neural crest-derived cells located at the presumptive dorsal root transitional zone (DRTZ) when sensory axons enter the spinal cord during development. Later, these cells migrate to dorsal root ganglia and differentiate into subtypes of sensory neurons and glia. After birth when the DRTZ is established, sensory axons are no longer able to enter the spinal cord. Here we explored the fate of mouse boundary cap neural crest stem cells (bNCSCs) implanted to the injured DRTZ after dorsal root avulsion for their potential to assist sensory axon regeneration. Grafted cells showed extensive survival and differentiation after transplantation to the avulsed DRTZ. Transplanted cells located outside the spinal cord organized elongated tubes of Sox2/GFAP expressing cells closely associated with regenerating sensory axons or appeared as small clusters on the surface of the spinal cord. Other cells, migrating into the host spinal cord as single cells, differentiated to spinal cord neurons with different neurotransmitter characteristics, extensive fiber organization, and in some cases surrounded by glutamatergic terminal-like profiles. These findings demonstrate that bNCSCs implanted at the site of dorsal root avulsion injury display remarkable differentiation plasticity inside the spinal cord and in the peripheral compartment where they organize tubes associated with regenerating sensory fibers. These properties offer a basis for exploring the ability of bNCSCs to assist regeneration of sensory axons into the spinal cord and replace lost neurons in the injured spinal cord.
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