Molecular cloning of lungfish proopiomelanocortin cDNA.

Molecular cloning of lungfish proopiomelanocortin cDNA.
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肺鱼阿黑皮质素原 cDNA 的分子克隆。

DOI:
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发表时间:
1999
影响因子:
2.7
通讯作者:
H. Kawauchi
H. Kawauchi
中科院分区:
医学3区
文献类型:
--
作者:
Y. Amemiya;A. Takahashi;H. Meguro;H. Kawauchi

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为了研究阿黑皮素原(proopiomelanocortin,POMC)在鱼类到四足动物中的进化,测定了非洲肺鱼POMC cDNA的核苷酸序列。从肺鱼脑垂体中制备POMC cDNA。POMC cDNA全长1114 bp,除poly-A尾外,编码255个氨基酸,其中信号肽25个。肺鱼POMC含有分别在位置(50-61)、(108-146)、(108 -120)、(178 -194)和(197-230)对应于γ-促黑素(MSH)、促肾上腺皮质素、α-MSH、β-MSH和β-内啡肽的片段。肺鱼POMC与两栖类(62%)、古鳍鱼类(62%)和哺乳类(52%)的序列同源性高于硬骨鱼类(49%)、板鳃类(46%)和无颌类(31%)。因此,肺鱼POMC的整体结构特征与含γ-MSH序列的四足动物POMC和含γ-MSH序列的古辐鳍鱼POMC接近。然而,肺鱼β-内啡肽的氨基酸序列表现出在鳍鱼和板鳃类中特别观察到的特性。
To investigate the evolution of proopiomelanocortin (POMC) from fish to tetrapods, nucleotide sequence of POMC cDNA from a lobe-finned fish, the African lungfish, was determined. POMC cDNA was prepared from lungfish pituitary glands. The POMC cDNA is composed of 1114 bp, excluding a poly-A tail, and encodes 255 amino acids (aa) including a signal peptide of 25 aa. The lungfish POMC contains the segment corresponding to gamma-melanotropin (MSH), corticotropin, alpha-MSH, beta-MSH, and beta-endorphin at positions (50-61), (108-146), (108-120), (178-194), and (197-230), respectively. The lungfish POMC shows greater sequence identity on average with amphibian (62%), ancient ray-finned fishes including acipenseriformes and semionotiformes (62%), and mammalian POMC (52%) than with teleostean (49%), elasmobranch (46%), and agnathan POMC (31%). Thus, the overall structural feature of lungfish POMC is close to the tetrapod POMCs which contain gamma-MSH and the ancient ray-finned fishes POMCs containing gamma-MSH-like sequence. However, amino acid sequence of lungfish beta-endorphin exhibits properties which are specifically observed in the ray-finned fishes and the elasmobranchs.
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发表时间: 1988
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影响因子: --
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DOI: --
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发表时间: 1990
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作者:
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