Domain movements of the enhancer-dependent sigma factor drive DNA delivery into the RNA polymerase active site: insights from single molecule studies.
Domain movements of the enhancer-dependent sigma factor drive DNA delivery into the RNA polymerase active site: insights from single molecule studies.
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DOI：
10.1093/nar/gku146
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发表时间：
2014-04
影响因子：
14.9
通讯作者：
Tuma R
中科院分区：
文献类型：
--
作者：
Sharma A;Leach RN;Gell C;Zhang N;Burrows PC;Shepherd DA;Wigneshweraraj S;Smith DA;Zhang X;Buck M;Stockley PG;Tuma R
细菌启动子的识别受两类不同的序列特异性Sigma因子调控，即σ70或σ54，它们的初级序列不同，后者通过增强子结合的上游激活子激活的要求也不同。σ54版本控制应激反应中的基因表达，通常介导致病性。它的激活蛋白是AAA+超家族的成员，利用三磷酸腺苷(ATP)的水解来重塑最初自我抑制的全酶启动子复合体。我们已经使用单分子荧光光谱绘制了这种重塑的图谱。最初的重塑是不依赖核苷酸的，由启动子熔化过程中的单链DNA和激活剂的结合驱动。然而，DNA到RNA聚合酶活性部位的装载依赖于激活剂对ATP的协同水解。虽然耦合的启动子识别和熔化步骤在σ70和σ54之间可能是保守的，但后者的结构域移动已经进化到需要激活ATPase。
Recognition of bacterial promoters is regulated by two distinct classes of sequence-specific sigma factors, σ70 or σ54, that differ both in their primary sequence and in the requirement of the latter for activation via enhancer-bound upstream activators. The σ54 version controls gene expression in response to stress, often mediating pathogenicity. Its activator proteins are members of the AAA+ superfamily and use adenosine triphosphate (ATP) hydrolysis to remodel initially auto-inhibited holoenzyme promoter complexes. We have mapped this remodeling using single-molecule fluorescence spectroscopy. Initial remodeling is nucleotide-independent and driven by binding both ssDNA during promoter melting and activator. However, DNA loading into the RNA polymerase active site depends on co-operative ATP hydrolysis by the activator. Although the coupled promoter recognition and melting steps may be conserved between σ70 and σ54, the domain movements of the latter have evolved to require an activator ATPase.
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影响因子：
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作者：
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作者：
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