Wnt/β-catenin signaling pathway regulates cell proliferation but not muscle dedifferentiation nor apoptosis during sea cucumber intestinal regeneration.
Wnt/β-catenin signaling pathway regulates cell proliferation but not muscle dedifferentiation nor apoptosis during sea cucumber intestinal regeneration.
复制标题
Wnt/β-catenin信号通路调控海参肠再生过程中的细胞增殖，但不调控肌肉去分化和细胞凋亡。
DOI：
10.1016/j.ydbio.2021.08.011
复制
发表时间：
2021-12
影响因子：
2.7
通讯作者：
Garcia-Arraras, Jose E.
中科院分区：
文献类型：
--
作者：
Alicea-Delgado, Miosotis;Garcia-Arraras, Jose E.
再生是生物体在受伤或疾病后恢复重要组织和器官成分的关键发育过程。人们越来越关注这些再生过程中涉及的分子机制的阐明和表征。我们现在分析了 Wnt/β-catenin 通路对海参肠道再生的可能作用。为此，我们研究了 Wnt 相关基因的体内表达，并使用 Dicer 底物干扰 RNA (DsiRNA) 来敲低肠道雏形外植体上 β-连环蛋白转录物的表达。肠道雏形外植体中β-连环蛋白转录本的减少不会影响细胞去分化和细胞凋亡。然而，干扰后增殖细胞的数量显着减少，表明Wnt/β-catenin信号通路在细胞增殖中发挥重要作用，但在肠道再生过程中的细胞去分化或凋亡中不发挥重要作用。体外 RNAi 方案的开发是分析棘皮动物再生中涉及的特定基因功能的重要一步。
Regeneration is a key developmental process by which organisms recover vital tissue and organ components following injury or disease. A growing interest is focused on the elucidation and characterization of the molecular mechanisms involved in these regenerative processes. We have now analyzed the possible role of the Wnt/β-catenin pathway on the regeneration of the intestine in the sea cucumber Holothuria glaberrima. For this we have studied the expression in vivo of Wnt-associated genes and have implemented the use of Dicer-substrate interference RNA (DsiRNA) to knockdown the expression of β-catenin transcript on gut rudiment explants. Neither cell dedifferentiation nor apoptosis were affected by the reduction of β-catenin transcripts in the gut rudiment explants. Yet, the number of proliferating cells decreased significantly following the interference, suggesting that the Wnt/β-catenin signaling pathway plays a significant role in cell proliferation, but not in cell dedifferentiation nor apoptosis during the regeneration of the intestine. The development of the in vitro RNAi protocol is a significant step in analyzing specific gene functions involved in echinoderm regeneration.
登录
查看更多内容
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
0.7
作者：
Iachetta, Roberta;Ambrosone, Alfredo;Tortiglione, Claudia
通讯作者：
Tortiglione, Claudia
影响因子：
--
作者：
García-Arrarás JE;Valentín-Tirado G;Flores JE;Rosa RJ;Rivera-Cruz A;San Miguel-Ruiz JE;Tossas K
通讯作者：
Tossas K
影响因子：
46.9
作者：
Kim, DH;Behlke, MA;Rossi, JJ
通讯作者：
Rossi, JJ
DOI：
10.1007/978-1-0716-0974-3_15
发表时间：
2021
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
通讯作者：
--