Assessment of mouse germinal vesicle stage oocyte quality by evaluating the cumulus layer, zona pellucida, and perivitelline space.
Assessment of mouse germinal vesicle stage oocyte quality by evaluating the cumulus layer, zona pellucida, and perivitelline space.
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通过评估卵丘层、透明带和卵周间隙来评估小鼠生殖囊泡阶段卵母细胞质量
DOI：
10.1371/journal.pone.0105812
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Liang CG
中科院分区：
文献类型：
--
作者：
Zhou HX;Ma YZ;Liu YL;Chen Y;Zhou CJ;Wu SN;Shen JP;Liang CG
To improve the outcome of assisted reproductive technology (ART) for patients with ovulation problems, it is necessary to retrieve and select germinal vesicle (GV) stage oocytes with high developmental potential. Oocytes with high developmental potential are characterized by their ability to undergo proper maturation, fertilization, and embryo development. In this study, we analyzed morphological traits of GV stage mouse oocytes, including cumulus cell layer thickness, zona pellucida thickness, and perivitelline space width. Then, we assessed the corresponding developmental potential of each of these oocytes and found that it varies across the range measured for each morphological trait. Furthermore, by manipulating these morphological traits in vitro, we were able to determine the influence of morphological variation on oocyte developmental potential. Manually altering the thickness of the cumulus layer showed strong effects on the fertilization and embryo development potentials of oocytes, whereas manipulation of zona pellucida thickness effected the oocyte maturation potential. Our results provide a systematic detailed method for selecting GV stage oocytes based on a morphological assessment approach that would benefit for several downstream ART applications.
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影响因子：
3.7
作者：
Choi JK;He X
通讯作者：
He X
影响因子：
6.1
作者：
Dunning, Kylie R.;Lane, Michelle;Russell, Darryl L.
通讯作者：
Russell, Darryl L.
影响因子：
4
作者：
Chamayou, S.;Ragolia, C.;Guglielmino, A.
通讯作者：
Guglielmino, A.
影响因子：
3.6
作者：
HOWARD, JG;DONOGHUE, AM;WILDT, DE
通讯作者：
WILDT, DE
影响因子：
--
作者：
Elvin, JA;Clark, AT;Matzuk, MM
通讯作者：
Matzuk, MM