Developmental expression of the glycine transporter GLYT2 in the auditory system of rats suggests involvement in synapse maturation
Developmental expression of the glycine transporter GLYT2 in the auditory system of rats suggests involvement in synapse maturation
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甘氨酸转运蛋白 GLYT2 在大鼠听觉系统中的发育表达表明参与突触成熟
DOI：
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
F. Zafra
中科院分区：
文献类型：
--
作者：
E. Friauf;C. Aragón;S. Löhrke;Beate Westenfelder;F. Zafra
许多神经递质的突触作用由特定的转运蛋白终止，转运蛋白从突触间隙中清除分子并帮助补充递质供应。在这里，我们研究了甘氨酸转运蛋白GLYT 2在大鼠中枢听觉系统的时空分布，甘氨酸能突触丰富。在成年大鼠中，GLYT 2免疫反应被发现在所有中继站，除了听觉皮层。在耳蜗核复合体、上级橄榄复合体和外侧丘系核中，神经元胞体周围有许多免疫反应点。相反，在下丘和内侧膝状体中观察到弥漫性神经纤维标记。点状体周标记和弥漫性神经毡标记与之前描述的听觉系统中甘氨酸抗体的染色模式非常相似，表明GLYT 2是甘氨酸能突触的可靠标记物。然而，细胞质GLYT 2和甘氨酸标记之间存在差异，因为并非所有先前用甘氨酸抗体鉴定的神经元类型都显示体细胞GLYT 2免疫反应性。在发育过程中，GLYT 2免疫反应性出现在胚胎第18天和第20天之间，即，在听觉系统中最早的功能性突触建立之后不久。标记从弥漫性模式转变为聚集的点状外观。发育的特征还在于信号强度的增加，其通常持续到出生后约10天。此后，减少发生，直到出生后第21天，当成熟的模式建立在大多数核。由于GLYT 2免疫反应性的围产期发病，我们推测转运蛋白分子参与早期突触成熟的过程。J. Comp.神经元412：17-37，1999.© 1999 Wiley‐利斯公司
The synaptic action of many neurotransmitters is terminated by specific transporters that remove the molecules from the synaptic cleft and help to replenish the transmitter supply. Here, we have investigated the spatiotemporal distribution of the glycine transporter GLYT2 in the central auditory system of rats, where glycinergic synapses are abundant. In adult rats, GLYT2 immunoreactivity was found at all relay stations, except the auditory cortex. Many immunoreactive puncta surrounded the neuronal somata in the cochlear nuclear complex, the superior olivary complex, and the nuclei of the lateral lemniscus. In contrast, diffuse neuropil labeling was seen in the inferior colliculus and the medial geniculate body. The punctate perisomatic labeling and the diffuse neuropil labeling were very similar to the staining pattern described previously with glycine antibodies in the auditory system, suggesting that GLYT2 is a reliable marker for glycinergic synapses. However, there was a discrepancy between cytoplasmic GLYT2 and glycine labeling, as not all neuron types previously identified with glycine antibodies displayed somatic GLYT2 immunoreactivity. During development, GLYT2 immunoreactivity appeared between embryonic days 18 and 20, i.e., shortly after the time when the earliest functional synapses have been established in the auditory system. Labeling turned from a diffuse pattern to a clustered, punctate appearance. The development was also characterized by an increase of the signal intensity, which generally lasted until about postnatal day 10. Thereafter, a decrease occurred until about postnatal day 21, when the mature pattern was established in most nuclei. Because of the perinatal onset of GLYT2 immunoreactivity, we speculate that the transporter molecules participate in the process of early synapse maturation. J. Comp. Neurol. 412:17–37, 1999. © 1999 Wiley‐Liss, Inc.
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影响因子：
2.5
作者：
Zhang,S;Oertel,D
通讯作者：
Oertel,D
DOI：
10.1073/pnas.89.15.7189
发表时间：
1992-08-01
影响因子：
11.1
作者：
GUASTELLA, J;BRECHA, N;DAVIDSON, N
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DAVIDSON, N
DOI：
10.1016/0169-328x(96)00079-4
发表时间：
1996-08
期刊：
Brain research. Molecular brain research
影响因子：
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作者：
D. Goebel
通讯作者：
D. Goebel
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DOI：
10.1152/jn.1995.73.4.1653
发表时间：
1995
期刊：
Journal of neurophysiology.
影响因子：
--
作者：
Smith,PH
通讯作者：
Smith,PH
影响因子：
3.6
作者：
Kyeong Man Kim;Stephen F. Kingsmore;H. Han;Teresa L. Yang-Feng;N. Godinot;Michael F. Seldin;M. G. Caron;Bruno Giros
通讯作者：
Kyeong Man Kim;Stephen F. Kingsmore;H. Han;Teresa L. Yang-Feng;N. Godinot;Michael F. Seldin;M. G. Caron;Bruno Giros