Identification and characterisation of isoprene-degrading bacteria in an estuarine environment.
Identification and characterisation of isoprene-degrading bacteria in an estuarine environment.
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DOI：
10.1111/1462-2920.13842
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发表时间：
2017-09
影响因子：
5.1
通讯作者：
Colin Murrell J
中科院分区：
文献类型：
--
作者：
Johnston A;Crombie AT;El Khawand M;Sims L;Whited GM;McGenity TJ;Colin Murrell J
排放到大气中的挥发性有机化合物（VOC）中约有三分之一由异戊二烯组成，来自陆地和海洋生物圈，对大气化学产生深远影响。然而，异戊二烯为微生物提供了丰富且基本上未开发的碳和能量来源。采用DNA稳定同位素探测（DNA-SIP）技术研究了英国科尔恩河口海洋和河口样品中异戊二烯降解的可能性。在两个时间点的分析表明，社区的发展占主导地位的放线菌，包括属分枝杆菌，红球菌，微杆菌和戈登氏菌的成员。从海洋和河口地区获得了能够以异戊二烯作为唯一碳源和能源生长的代表性分离株，并对戈登氏菌和分枝杆菌的异戊二烯降解菌株进行了生理学表征，并对其基因组进行了测序。预测异戊二烯代谢所需的基因，包括四组分异戊二烯单加氧酶（IsoMO），被鉴定并与先前表征的实例进行比较。异戊二烯或替代碳源上生长的菌株的转录和活性测定表明，异戊二烯上的生长是需要特定IsoMO的诱导性状。这项研究是第一个使用DNA-SIP在河口和海洋环境中鉴定活性异戊二烯降解菌，并在生理和分子水平上鉴定海洋异戊二烯降解菌。
Approximately one‐third of volatile organic compounds (VOCs) emitted to the atmosphere consists of isoprene, originating from the terrestrial and marine biosphere, with a profound effect on atmospheric chemistry. However, isoprene provides an abundant and largely unexplored source of carbon and energy for microbes. The potential for isoprene degradation in marine and estuarine samples from the Colne Estuary, UK, was investigated using DNA‐Stable Isotope Probing (DNA‐SIP). Analysis at two timepoints showed the development of communities dominated by Actinobacteria including members of the genera Mycobacterium, Rhodococcus, Microbacterium and Gordonia. Representative isolates, capable of growth on isoprene as sole carbon and energy source, were obtained from marine and estuarine locations, and isoprene‐degrading strains of Gordonia and Mycobacterium were characterised physiologically and their genomes were sequenced. Genes predicted to be required for isoprene metabolism, including four‐component isoprene monooxygenases (IsoMO), were identified and compared with previously characterised examples. Transcriptional and activity assays of strains growing on isoprene or alternative carbon sources showed that growth on isoprene is an inducible trait requiring a specific IsoMO. This study is the first to identify active isoprene degraders in estuarine and marine environments using DNA‐SIP and to characterise marine isoprene‐degrading bacteria at the physiological and molecular level.
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影响因子：
6.5
作者：
Capone, Kimberly A.;Dowd, Scot E.;Stamatas, Georgios N.;Nikolovski, Janeta
通讯作者：
Nikolovski, Janeta
影响因子：
6.3
作者：
Booge, Dennis;Marandino, Christa A.;Wallace, Douglas W. R.
通讯作者：
Wallace, Douglas W. R.
影响因子：
5.2
作者：
Broadgate, WJ;Liss, PS;Penkett, SA
通讯作者：
Penkett, SA
影响因子：
5.1
作者：
El Khawand, Myriam;Crombie, Andrew T.;Murrell, J. Colin
通讯作者：
Murrell, J. Colin
影响因子：
4.4
作者：
Gilbert, B;McDonald, IR;Murrell, JC
通讯作者：
Murrell, JC