Generation of a tumor spheroid in a microgravity environment as a 3D model of melanoma.
Generation of a tumor spheroid in a microgravity environment as a 3D model of melanoma.
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DOI：
10.1007/s11626-009-9217-2
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发表时间：
2009-10
影响因子：
2.1
通讯作者：
Heller, Richard
中科院分区：
文献类型：
--
作者：
Marrero, Bernadette;Messina, Jane L.;Heller, Richard
利用合成微重力环境开发了体外3D模型，以便于研究细胞相互作用。2D单层细胞培养模型已成功用于了解体内发生的各种细胞反应。与在3D基质中生长的细胞相比，2D模型存在一些明显的局限性。例如，一些在2D模型中不表达的蛋白质在3D矩阵中被发现上调。在本文中，我们讨论了用于开发第一个已知的大型，自由浮动的3D组织模型，用于建立肿瘤球体的技术。被称为高纵横比容器（HARVs）的生物反应器系统用于提供微重力环境。HARV促进角质形成细胞（HaCaT）的聚集，所述角质形成细胞（HaCaT）形成充当小鼠黑色素瘤生长的支架的构建体。虽然有一个重点是建立一个3D模型与适当的细胞外基质和基质，我们能够开发一个模型，排除使用matrigel。免疫组织化学和凋亡测定提供了证据，该3D模型支持B16 F10细胞生长、增殖和细胞外基质合成。免疫荧光显示，黑色素瘤细胞相互作用，显示可观察到的细胞形态学变化。设计3D组织模型的目的是收集有关癌症发展的新信息，并开发新的潜在治疗方案，这些方案可以转化为体内模型，同时减少实验室动物的使用。
An in vitro 3D model was developed utilizing a synthetic microgravity environment to facilitate studying the cell interactions. 2D monolayer cell culture models have been successfully used to understand various cellular reactions that occur in vivo. There are some limitations to the 2D model that are apparent when compared to cells grown in a 3D matrix. For example, some proteins that are not expressed in a 2D model are found up-regulated in the 3D matrix. In this paper, we discuss techniques used to develop the first known large, free-floating 3D tissue model used to establish tumor spheroids. The bioreactor system known as the High Aspect Ratio Vessel (HARVs) was used to provide a microgravity environment. The HARVs promoted aggregation of keratinocytes (HaCaT) that formed a construct that served as scaffolding for the growth of mouse melanoma. Although there’s an emphasis on building a 3D model with the proper extracellular matrix and stroma, we were able to develop a model that excluded the use of matrigel. Immunohistochemistry and apoptosis assays provided evidence that this 3D model supports B16F10 cell growth, proliferation and synthesis of extracellular matrix. Immunofluorescence showed that melanoma cells interact with one another displaying observable cellular morphological changes. The goal of engineering a 3D tissue model is to collect new information about cancer development and develop new potential treatment regimens that can be translated to in vivo models while reducing the use of laboratory animals.
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影响因子：
2.7
作者：
Nakamura, K;Kuga, H;Katano, M
通讯作者：
Katano, M
影响因子：
48
作者：
Fischbach, Claudia;Chen, Ruth;Mooney, David J.
通讯作者：
Mooney, David J.
影响因子：
4
作者：
Hindie, M.;Vayssade, M.;Nagel, M. -D.
通讯作者：
Nagel, M. -D.
DOI：
10.1007/bf01404744
发表时间：
1992-01-01
期刊：
Journal of tissue culture methods : Tissue Culture Association manual of cell, tissue, and organ culture procedures
影响因子：
--
作者：
Schwarz, R P;Goodwin, T J;Wolf, D A
通讯作者：
Wolf, D A
影响因子：
6
作者：
Hsu, MY;Meier, FE;Herlyn, M
通讯作者：
Herlyn, M