Requirement of Zebrafish Adcy3a and Adcy5 in Melanosome Dispersion and Melanocyte Stripe Formation.
Requirement of Zebrafish Adcy3a and Adcy5 in Melanosome Dispersion and Melanocyte Stripe Formation.
复制标题
斑马鱼Adcy3a和Adcy5在黑色素小体分散和黑素细胞条纹形成中的需求。
DOI：
10.3390/ijms232214182
复制
发表时间：
2022-11-16
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
cAMP-PKA signaling plays a pivotal role in melanin synthesis and melanosome transport by responding to the binding of the α-melanocyte-stimulating hormone (α-MSH) to melanocortin-1 receptor (MC1R). Adenylate cyclases (ADCYs) are the enzymes responsible for the synthesis of cAMP from ATP, which comprises nine transmembrane isoforms (ADCYs 1-9) and one soluble adenylate cyclase (ADCY 10) in mammals. However, little is known about which and how ADCY isoforms regulate melanocyte generation, melanin biosynthesis, and melanosome transport in vivo. In this study, we have generated a series of single and double mutants of Adcy isoforms in zebrafish. Among them, adcy3a-/- and adcy5-/- double mutants cause defects in melanosome dispersion but do not impair melanoblast differentiation and melanocyte regeneration during the embryonic or larval stages. Activation of PKA, the main effector of cAMP signaling, significantly ameliorates the defects in melanosome dispersion in adcy3a-/- and adcy5-/- double mutants. Mechanistically, Adcy3a and Adcy5 regulate melanosome dispersion by activating kinesin-1 while inhibiting cytoplasmic dynein-1. In adult zebrafish, Adcy3a and Adcy5 participate in the regulation of the expression of microphthalmia transcription factor (Mitfa) and melanin synthesis enzymes Tyr, Dct, and Trp1b. The deletion of Adcy3a and Adcy5 inhibits melanin production and reduces pigmented melanocyte numbers, causing a defect in establishing adult melanocyte stripes. Hence, our studies demonstrate that Adcy3a and Adcy5 play essential but redundant functions in mediating α-MSH-MC1R/cAMP-PKA signaling for regulating melanin synthesis and melanosome dispersion.
登录
查看更多内容
影响因子：
9.9
作者：
Harms, M. B.;Ori-McKenney, K. M.;Baloh, R. H.
通讯作者：
Baloh, R. H.
DOI：
10.1083/jcb.117.1.57
发表时间：
1992-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Sammak PJ;Adams SR;Harootunian AT;Schliwa M;Tsien RY
通讯作者：
Tsien RY
影响因子：
21.3
作者：
通讯作者：
--
DOI：
10.1111/j.1600-0749.2006.00307.x
发表时间：
2006-06-01
期刊：
PIGMENT CELL RESEARCH
影响因子：
--
作者：
Logan, Darren W.;Burn, Sally F.;Jackson, Ian J.
通讯作者：
Jackson, Ian J.
影响因子：
4.6
作者：
Elworthy, S;Lister, JA;Kelsh, RN
通讯作者：
Kelsh, RN