A Bat-Derived Putative Cross-Family Recombinant Coronavirus with a Reovirus Gene.
A Bat-Derived Putative Cross-Family Recombinant Coronavirus with a Reovirus Gene.
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具有呼肠孤病毒基因的蝙蝠衍生的假定跨家族重组冠状病毒。
DOI：
10.1371/journal.ppat.1005883
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发表时间：
2016-09
期刊：
影响因子：
6.7
通讯作者：
Gao GF
中科院分区：
文献类型：
--
作者：
Huang C;Liu WJ;Xu W;Jin T;Zhao Y;Song J;Shi Y;Ji W;Jia H;Zhou Y;Wen H;Zhao H;Liu H;Li H;Wang Q;Wu Y;Wang L;Liu D;Liu G;Yu H;Holmes EC;Lu L;Gao GF
2002年出现的严重急性呼吸综合征冠状病毒（SARS-CoV）和2012年出现的中东呼吸综合征冠状病毒（MERS-CoV）引起了人们对冠状病毒的生物多样性、基因组学和跨物种传播潜力的极大兴趣，特别是来自蝙蝠的冠状病毒，蝙蝠是哺乳动物中物种第二多的目。本研究利用泛冠状病毒RT-PCR和新一代测序技术，从leschenaulti Rousettus bat直肠拭子样本中鉴定出一种新型冠状病毒，暂命名为Rousettus bat冠状病毒GCCDC1 （Ro-BatCoV GCCDC1）。虽然该病毒在基因组特征上与蝙蝠冠状病毒HKU9 （Ro-BatCoV HKU9）相似，但根据国际病毒分类委员会（ICTV）定义的标准，该病毒具有足够的差异性，可被归类为新种。更令人惊讶的是，Ro-BatCoV GCCDC1包含一个独特的基因，整合到基因组的3 '端，在任何已知的冠状病毒中都没有同源物，但序列和系统发育分析表明，该基因最有可能来自蝙蝠正呼肠孤病毒的p10基因。亚基因组mRNA和细胞水平的观察表明，p10基因是功能性的，并诱导细胞合胞体的形成。因此，在这里，我们报道了单链阳性RNA病毒和双链分段RNA病毒之间的异源家族间重组事件，为病毒进化的基本机制提供了见解。重组是冠状病毒中普遍报道的，是这些病毒产生遗传多样性的重要机制。然而，迄今为止，大多数此类重组事件涉及相关病毒之间的同源序列。我们发现了一种新的蝙蝠冠状病毒，它具有不同的但功能性的p10基因，可能起源于祖先的非包膜正呼肠孤病毒，或与之有共同的祖先，因此代表了异源重组的结果。我们在此报告了一种被包膜病毒中编码的融合相关小跨膜（FAST）蛋白，该蛋白是通过单链阳性RNA病毒和双链分段RNA病毒之间假定的家族间重组而产生的。这些发现揭示了病毒进化的机制，特别是异源重组的重要性和范围。
The emergence of severe acute respiratory syndrome coronavirus (SARS-CoV) in 2002 and Middle East respiratory syndrome coronavirus (MERS-CoV) in 2012 has generated enormous interest in the biodiversity, genomics and cross-species transmission potential of coronaviruses, especially those from bats, the second most speciose order of mammals. Herein, we identified a novel coronavirus, provisionally designated Rousettus bat coronavirus GCCDC1 (Ro-BatCoV GCCDC1), in the rectal swab samples of Rousettus leschenaulti bats by using pan-coronavirus RT-PCR and next-generation sequencing. Although the virus is similar to Rousettus bat coronavirus HKU9 (Ro-BatCoV HKU9) in genome characteristics, it is sufficiently distinct to be classified as a new species according to the criteria defined by the International Committee of Taxonomy of Viruses (ICTV). More striking was that Ro-BatCoV GCCDC1 contained a unique gene integrated into the 3’-end of the genome that has no homologs in any known coronavirus, but which sequence and phylogeny analyses indicated most likely originated from the p10 gene of a bat orthoreovirus. Subgenomic mRNA and cellular-level observations demonstrated that the p10 gene is functional and induces the formation of cell syncytia. Therefore, here we report a putative heterologous inter-family recombination event between a single-stranded, positive-sense RNA virus and a double-stranded segmented RNA virus, providing insights into the fundamental mechanisms of viral evolution. Recombination is commonly reported in coronaviruses, and is an important mechanism by which these viruses generate genetic diversity. To date, however, most such recombination events involve homologous sequences among related viruses. We discovered a novel bat coronavirus that possesses a divergent but functional p10 gene that likely originated from, or shared the ancestry with, an ancestral non-enveloped orthoreovirus, thereby representing the outcome of heterologous recombination. We report herein a fusion-associated small transmembrane (FAST) protein encoded in an enveloped virus that arose through a putative inter-family recombination between a single-stranded, positive-sense RNA virus and a double-stranded segmented RNA virus. These findings shed important new light on the mechanisms of viral evolution and particularly the importance and scope of heterologous recombination.
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影响因子：
5.4
作者：
Fang, Shouguo;Shen, Hongyuan;Liu, Ding Xiang
通讯作者：
Liu, Ding Xiang
影响因子：
5.4
作者：
Corman, Victor Max;Baldwin, Heather J.;Drexler, Jan Felix
通讯作者：
Drexler, Jan Felix
影响因子：
5.4
作者：
Cornelissen, LAHM;Wierda, CMH;deGroot, RJ
通讯作者：
deGroot, RJ
影响因子：
--
作者：
Duan, Q;Zhu, H;Zhang, XM
通讯作者：
Zhang, XM
DOI：
10.1107/s0907444998003254
发表时间：
1998-09-01
期刊：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
影响因子：
--
作者：
Brunger, AT;Adams, PD;Warren, GL
通讯作者：
Warren, GL