Mutations in Arabidopsis YCF20-like genes affect thermal dissipation of excess absorbed light energy.
Mutations in Arabidopsis YCF20-like genes affect thermal dissipation of excess absorbed light energy.
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DOI：
10.1007/s00425-010-1098-9
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发表时间：
2010-03
期刊：
影响因子：
4.3
通讯作者：
Niyogi KK
中科院分区：
文献类型：
--
作者：
Jung HS;Niyogi KK
植物将吸收的多余光能转化为热量，以保护自身免受光氧化胁迫。拟南芥（Arabidopsis thaliana）npq 6突变体在热耗散方面受到影响，其部分缺陷在于过量光诱导叶绿素荧光（NPQ）的非光化学猝灭。定位克隆显示npq 6含有由At 5g 43050基因中的单个碱基对缺失引起的移码突变，At 5g 43050基因编码具有未知功能的假定叶绿体开放阅读框架20（YCF 20）蛋白家族的成员。YCF 20蛋白家族主要在含氧光合生物中保守，包括蓝藻、真核藻类和植物。氨基酸序列比较确定了拟南芥中编码与NPQ 6相似蛋白的另外两个基因：At 1g 65420和At 3g 56830。这三个拟南芥蛋白具有功能性叶绿体靶向转运肽。使用反向遗传学，在At 1g 65420基因内插入T-DNA的突变体被鉴定，并显示出与npq 6相似的低NPQ表型;因此，At 1g 65420被命名为NPQ 7。相反，At 3g 56830基因中具有较低转录水平的敲低突变体显示出野生型水平的NPQ。npq 6和npq 7双突变体具有加性NPQ缺陷，表明拟南芥中的YCF 20家族成员具有影响热耗散的重叠功能。本文的在线版本（doi：10.1007/s 00425 -010-1098-9）包含补充材料，可供授权用户使用。
Plants dissipate excess absorbed light energy as heat to protect themselves from photo-oxidative stress. The Arabidopsis thaliana npq6 mutant affected in thermal dissipation was identified by its partial defect in the induction of nonphotochemical quenching of chlorophyll fluorescence (NPQ) by excess light. Positional cloning revealed that npq6 contains a frameshift mutation caused by a single base-pair deletion in the At5g43050 gene, which encodes a member of the hypothetical chloroplast open reading frame 20 (YCF20) family of proteins with unknown function(s). The YCF20 protein family is mostly conserved in oxygenic photosynthetic organisms including cyanobacteria, eukaryotic algae, and plants. Amino acid sequence comparison identified two other genes in Arabidopsis that encode similar proteins to NPQ6: At1g65420 and At3g56830. These three Arabidopsis proteins have functional chloroplast-targeting transit peptides. Using reverse genetics, a mutant with a T-DNA insertion within the At1g65420 gene was identified and shown to exhibit a low NPQ phenotype similar to that of npq6; therefore, At1g65420 was named NPQ7. In contrast, a knockdown mutant in the At3g56830 gene with lower transcript levels showed wild-type levels of NPQ. The npq6 npq7 double mutant had an additive NPQ defect, indicating that the YCF20 family members in Arabidopsis have overlapping functions affecting thermal dissipation. The online version of this article (doi:10.1007/s00425-010-1098-9) contains supplementary material, which is available to authorized users.
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