Minichromosome Maintenance (MCM) Family as potential diagnostic and prognostic tumor markers for human gliomas.
Minichromosome Maintenance (MCM) Family as potential diagnostic and prognostic tumor markers for human gliomas.
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微型染色体维持（MCM）家族作为人类神经胶质瘤的潜在诊断和预后肿瘤标志物
DOI：
10.1186/1471-2407-14-526
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发表时间：
2014-07-21
期刊：
影响因子：
3.8
通讯作者：
Bie L
中科院分区：
文献类型：
--
作者：
Hua C;Zhao G;Li Y;Bie L
神经胶质瘤是所有中枢神经系统肿瘤中最常见的类型。几乎所有被诊断患有这些肿瘤的患者预后都很差。我们的目的是鉴定新的神经胶质瘤预后相关候选基因。方法应用webbarraydb软件对微阵列数据集进行跨平台集成和分析。我们专注于显著上调基因的一个子集，即小染色体维持（MCM）家族。我们使用冷冻胶质瘤样本，通过qPCR和western blot预测mcm的表达与患者预后的关系。结果我们发现mcm在胶质瘤中表达显著上调。MCM2-7和MCM10的表达与WHO肿瘤分级相关。高MCM2 mRNA表达似乎与高级别胶质瘤患者较差的总生存率密切相关。此外，我们报道MCM7与WHO II-IV级肿瘤患者的预后密切相关。MCM3在胶质瘤中表达上调，并与WHO III级肿瘤患者的总生存率相关。根据目前WHO的分类系统，MCM2、MCM3和MCM7表达水平与预后的相关性大于组织学诊断。结论mcm2、MCM3和MCM7 mRNA的高表达与胶质瘤预后不良相关，可能是临床上有用的分子预后标志物。
BackgroundGliomas are the most common type of all central nervous system tumors. Almost all patients diagnosed with these tumors have a poor prognostic outcome. We aimed to identify novel glioma prognosis-associated candidate genes.MethodsWe applied WebArrayDB software to span platform integrate and analyze the microarray datasets. We focused on a subset of the significantly up-regulated genes, the minichromosome maintenance (MCM) family. We used frozen glioma samples to predict the relationship between the expression of MCMs and patients outcome by qPCR and western blot.ResultsWe found that MCMs expression was significantly up-regulated in glioma samples. MCM2-7 and MCM10 expressions were associated with WHO tumor grade. High MCM2 mRNA expression appeared to be strongly associated with poor overall survival in patients with high grade glioma. Furthermore, we report that MCM7 is strongly correlated with patient outcome in patients with WHO grade II-IV tumor. MCM3 expression was found to be up-regulated in glioma and correlated with overall survival in patients with WHO grade III tumor. MCM2, MCM3 and MCM7 expression levels were of greater prognostic relevance than histological diagnosis according to the current WHO classification system.ConclusionsHigh expression of MCM 2, MCM3 and MCM7 mRNA correlated with poor outcome and may be clinically useful molecular prognostic markers in glioma.
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非固定上皮卵巢肿瘤中的微小浓度维持蛋白2和5：与细胞周期调节剂和预后意义的关系。
影响因子：
8.8
作者：
Gakiopoulou, H;Korkolopoulou, P;Levidou, G;Thymara, I;Saetta, A;Piperi, C;Givalos, N;Vassilopoulos, I;Ventouri, K;Tsenga, A;Bamias, A;Dimopoulos, M-A;Agapitos, E;Patsouris, E
通讯作者：
Patsouris, E
影响因子：
24.5
作者：
Going, JJ;Keith, WN;Williams, GH
通讯作者：
Williams, GH
影响因子：
11.5
作者：
Ha, SA;Shin, SM;Kim, JW
通讯作者：
Kim, JW
影响因子：
5
作者：
Hashimoto, K;Araki, K;Ito, H
通讯作者：
Ito, H
DOI：
10.1046/j.1432-1033.2003.03440.x
发表时间：
2003-03-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Ishimi, Y;Okayasu, I;Song, SY
通讯作者：
Song, SY