Identification, structural determination, and biological activity of bovine and canine calcitonin receptor-stimulating peptides.
Identification, structural determination, and biological activity of bovine and canine calcitonin receptor-stimulating peptides.
复制标题
牛和犬降钙素受体刺激肽的鉴定、结构测定和生物活性。
DOI：
10.1016/j.bbrc.2003.11.114
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发表时间：
2004
影响因子：
3.1
通讯作者：
N. Minamino
中科院分区：
文献类型：
--
作者：
T. Katafuchi;K. Hamano;N. Minamino
我们最近在猪脑中发现了一系列新的肽，命名为降钙素受体刺激肽-1（CRSP-1），CRSP-2和CRSP-3，但通过数据库搜索或实验交叉杂交未能在人类和啮齿动物中找到它们的对应物。在这项研究中，我们从牛CRSP-1，犬CRSP-1和犬CRSP-2的甲状腺cDNA文库中分离出编码CRSP-1，CRSP-1和CRSP-2前体的cDNA。尽管推导的牛和犬CRSP-1和犬CRSP-2的成熟氨基酸序列与其各自的猪CRSP对应物显示出同一性，但它们都不具有C-末端酰胺结构。在内源性表达降钙素（CT）受体的LLC-PK 1细胞中，牛和犬CRSP-1可增强cAMP的产生，而犬CRSP-2则完全不刺激cAMP的产生。马CGRP-I在其氨基酸序列上与猪CRSP-1具有高度同源性，并且以与猪CT相当的效力刺激LLC-PK 1细胞。这些CRSP或马CGRP-I刺激CT样受体，即使在受体活性修饰蛋白的存在下。这些结果表明，CRSP-1，一类新的生物活性肽，存在于进化上接近猪的动物中，并通过降钙素受体诱导其活性，表明该肽在哺乳动物中广泛存在和共同特性。
We have recently identified in porcine brain a series of new peptides, designated calcitonin receptor-stimulating peptide-1 (CRSP-1), CRSP-2, and CRSP-3, but failed to find their counterparts in humans and rodents by either database searching or experimental cross-hybridization. In this study, we isolated cDNAs encoding precursors of bovine CRSP-1, canine CRSP-1, and canine CRSP-2 from their thyroid cDNA libraries. Although the deduced mature amino acid sequences of bovine and canine CRSP-1s and canine CRSP-2 showed identity with their respective porcine CRSP counterparts, none of them had a C-terminal amide structure. In LLC-PK1cells endogenously expressing the calcitonin (CT) receptor, bovine and canine CRSP-1s enhanced the cAMP production, while canine CRSP-2 did not stimulate it at all. Equine CGRP-I had a high identity in its amino acid sequence with porcine CRSP-1 and stimulated LLC-PK1cells at a potency comparable to that of porcine CT. None of these CRSPs or equine CGRP-I stimulated the CT-like receptor, even in the presence of receptor activity-modifying proteins. These results demonstrate that CRSP-1, a new class of biologically active peptide, is present in animals evolutionarily close to pigs and induces its activity through the calcitonin receptor, suggesting a wide existence and common properties of this peptide in mammals.
影响因子：
56.9
作者：
LIN, HY;HARRIS, TL;GOLDRING, SR
通讯作者：
GOLDRING, SR