Phenotypic and Genotypic Characterization of Streptococcus mutans Strains Isolated from Endodontic Infections.
Phenotypic and Genotypic Characterization of Streptococcus mutans Strains Isolated from Endodontic Infections.
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DOI：
10.1016/j.joen.2020.09.002
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发表时间：
2020-12
影响因子：
4.2
通讯作者：
Abranches J
中科院分区：
文献类型：
--
作者：
Lima AR;Ganguly T;Walker AR;Acosta N;Francisco PA;Pileggi R;Lemos JA;Gomes BPFA;Abranches J
变形链球菌在龋齿病因学和最终全身性感染中起重要作用。然而，它经常在感染的根管中发现，但居住在该部位的菌株的病理生理特征在很大程度上是未知的。在这里，我们研究了从原发性（PI）和继发性/持续性（SI）根管感染中分离的变形链球菌菌株的特征：血清型和基因型；胶原结合蛋白（CBPs）编码基因的存在；胶原结合活性和生物膜形成能力；能够承受环境压力；mellonella的系统毒力；人冠状动脉内皮细胞（HCAECs）和人牙蛹成纤维细胞（HDPFs）的侵袭。收集了10例PI患者和10例SI患者的样本，共分离出3种基因型的变形链球菌14株。其中c型13株，k型1株，临床分离株对双氧水过敏。值得注意的是，与cbp阴性菌株相比，所有14株菌株都携带并表达了cbp编码基因cbm，显示出与胶原蛋白的结合增加，对大蜡杆菌的全身毒力增强，并且能够侵入HCAECs和HDPFs。PI和SI分离株的全基因组序列分析表明，这两株在系统发育上是相关的，但在遗传上是不同的。我们的研究结果强调了CBPs在促进变形链球菌在胶原基质（如根管）中的定植和持久性方面的重要性，以及它们在牙髓感染发病机制中的潜在作用。
Streptococcus mutans plays an important role in caries etiology and eventually in systemic infections. However, it is often found in infected root canals but the pathophysiological characteristics of strains residing in this site are largely unkonwn. Here, we characterized strains of S. mutans isolated from root canals of primary (PI) and secondary/persistent (SI) endodontic infections in relation to: serotype and genotype; presence of genes coding for collagen binding proteins (CBPs); collagen binding activity and biofilm formation capacity; ability to withstand environmental stresses; systemic virulence in Galleria mellonella; invasion of human coronary artery endothelial cells (HCAECs) and human dental pupal fibroblasts (HDPFs). Samples from 10 patients with PI and 10 patients with SI were collected and a total of 14 S. mutans isolates, belonging to three genotypes, were obtained. Of these, 13 were serotype c and 1 was serotype k. When compared to the reference strains, the clinical isolates were hypersensitive to hydrogen peroxide. Remarkably, all 14 strains harbored and expressed the CBP-encoding gene cbm showing increased binding to collagen, enhanced systemic virulence in G. mellonella and ability to invade HCAECs and HDPFs when compared to CBP-negative strains. Whole genome sequence analysis of PI and SI isolates revealed that these strains are phylogenetically related but genetically distinct from each other. Our findings highlight the importance CBPs in facilitating colonization and persistence of S. mutans in collagenous substrates such as root canals and their potential role in the pathogenesis of endodontic infections.
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影响因子：
2.9
作者：
Valdez RMA;Duque C;Caiaffa KS;Dos Santos VR;Loesch MLA;Colombo NH;Arthur RA;Negrini TC;Boriollo MFG;Delbem ACB
通讯作者：
Delbem ACB
影响因子：
5.2
作者：
Freires, Irlan A.;Aviles-Reyes, Alejandro;Abranches, Jacqueline
通讯作者：
Abranches, Jacqueline
影响因子：
3.7
作者：
Abranches J;Zeng L;Kajfasz JK;Palmer SR;Chakraborty B;Wen ZT;Richards VP;Brady LJ;Lemos JA
通讯作者：
Lemos JA
影响因子：
4.2
作者：
Moses, Peter J.;Power, Daniel A.;Vickerman, M. Margaret
通讯作者：
Vickerman, M. Margaret
影响因子：
9.4
作者：
Nakano, K;Nomura, R;Ooshima, T
通讯作者：
Ooshima, T