Genotypic diversity and phenotypic traits of Streptococcus mutans isolates and their relation to severity of early childhood caries.
Genotypic diversity and phenotypic traits of Streptococcus mutans isolates and their relation to severity of early childhood caries.
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DOI：
10.1186/s12903-017-0406-1
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发表时间：
2017-07-14
期刊：
影响因子：
2.9
通讯作者：
Delbem ACB
中科院分区：
文献类型：
--
作者：
Valdez RMA;Duque C;Caiaffa KS;Dos Santos VR;Loesch MLA;Colombo NH;Arthur RA;Negrini TC;Boriollo MFG;Delbem ACB
幼儿龋齿（ECC）是一种侵袭性疾病，可影响幼儿的牙齿。本研究旨在评价S.从不同龋病状态的儿童与无龋（CF）儿童的牙齿生物膜中分离的变形杆菌进行比较。 从CF、ECC和重度ECC（S-ECC）儿童的龈上生物膜样品中分离变形链球菌菌株，并采用任意引物聚合酶链反应-AP-PCR进行基因分型。S.测试了变异株基因型通过糖酵解降低悬浮液pH的能力、耐受极端酸挑战的能力以及形成生物膜的能力。通过ANOVA/Tukey或Kruskal-Wallis/Mann-Whitney检验在5%的显著性下分析响应变量。生物膜中变形链球菌的检出率随龋病严重程度的增加而增加。CF、ECC和S-ECC儿童的基因型多样性和基因型产酸性无差异。ECC和S-ECC儿童的基因型特征的变形链球菌菌株比CF儿童形成更多的生物膜。从S-ECC患儿中分离的菌株耐酸性强。虽然S.变形链球菌基因型多样性在各组儿童中相似，变形链球菌表型特征相似。变形菌，特别是酸耐受反应，可以解释儿童早期龋的严重性。
Early childhood caries (ECC) is an aggressive condition that can affect teeth of young children. This study aimed to evaluate genotypic diversity and phenotypic traits of S. mutans isolated from dental biofilms of children with different caries status in comparison with caries free (CF) children. Streptococcus mutans strains were isolated from supragingival biofilm samples of CF, ECC and severe-ECC (S-ECC) children and genotyped by arbitrary-primer polymerase chain reaction - AP-PCR. S. mutans genotypes were tested for their ability to reduce the suspension pH through glycolysis, to tolerate extreme acid challenge and by their ability to form biofilm. Response variables were analyzed by ANOVA/Tukey or Kruskal-Wallis/Mann-Whitney tests at a 5% of significance. There was an increase in the prevalence of Streptococcus mutans in biofilms with the severity of dental caries. No differences in genotypic diversity and in acidogenicity of genotypes were found among CF, ECC and S-ECC children. S mutans strains with genotypes more characteristic for ECC and S-ECC children formed more biofilms than those identified in CF children. The strains isolated from S-ECC children were highly acid tolerant. Although S. mutans genotypic diversity was similar among the groups of children, phenotypic traits of S. mutans, especially the acid tolerance response, could explain the severity of early childhood caries.
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影响因子：
1.9
作者：
Cheon K;Moser SA;Wiener HW;Whiddon J;Momeni SS;Ruby JD;Cutter GR;Childers NK
通讯作者：
Childers NK
影响因子：
3
作者：
ALALUUSUA, S;ALALUUSUA, SJ;ASIKAINEN, S
通讯作者：
ASIKAINEN, S
影响因子：
7.6
作者：
Cheon, K.;Moser, S. A.;Childers, N. K.
通讯作者：
Childers, N. K.
影响因子：
3.1
作者：
BENDER, GR;SUTTON, SVW;MARQUIS, RE
通讯作者：
MARQUIS, RE
影响因子：
2.7
作者：
Tabchoury, Cínthia Pereira Machado;Sousa, Maria Clara K.;Cury, Jaime Aparecido
通讯作者：
Cury, Jaime Aparecido