Systematic characterization of gene function in the photosynthetic alga Chlamydomonas reinhardtii.
Systematic characterization of gene function in the photosynthetic alga Chlamydomonas reinhardtii.
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DOI：
10.1038/s41588-022-01052-9
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发表时间：
2022-05
期刊：
影响因子：
30.8
通讯作者：
Jinkerson, Robert E.
中科院分区：
文献类型：
--
作者：
Fauser, Friedrich;Vilarrasa-Blasi, Josep;Onishi, Masayuki;Ramundo, Silvia;Patena, Weronika;Millican, Matthew;Osaki, Jacqueline;Philp, Charlotte;Nemeth, Matthew;Salome, Patrice A.;Li, Xiaobo;Wakao, Setsuko;Kim, Rick G.;Kaye, Yuval;Grossman, Arthur R.;Niyogi, Krishna K.;Merchant, Sabeeha S.;Cutler, Sean R.;Walter, Peter;Dinneny, Jose R.;Jonikas, Martin C.;Jinkerson, Robert E.
光合生物中的大多数基因在功能上仍然没有特征。在这里，使用模式真核生物莱茵衣藻的条形码突变库，我们在超过121种不同的环境生长条件和化学处理下确定了超过58，000种突变体的表型。总共有59%的基因由至少一个表现出表型的突变体代表，为数千个基因的功能提供了线索。突变表型谱将未表征的基因置于功能途径中，如DNA修复、光合作用、CO2浓缩机制和纤毛发生。我们通过验证表型和基因功能来说明这种资源的价值，包括肌动蛋白细胞骨架防御途径的三个新组成部分。这些数据也为陆地植物的表型发现提供了信息;拟南芥基因的突变体表现出与我们在衣原体同源物中观察到的表型相似的表型。我们预计，这一资源将指导整个生命之树的基因的功能表征。在121种环境和化学胁迫条件下，对模式真核生物莱茵衣藻的58，101个突变体进行系统表型分析，为表征基因功能提供了大量资源。
Most genes in photosynthetic organisms remain functionally uncharacterized. Here, using a barcoded mutant library of the model eukaryotic alga Chlamydomonas reinhardtii, we determined the phenotypes of more than 58,000 mutants under more than 121 different environmental growth conditions and chemical treatments. A total of 59% of genes are represented by at least one mutant that showed a phenotype, providing clues to the functions of thousands of genes. Mutant phenotypic profiles place uncharacterized genes into functional pathways such as DNA repair, photosynthesis, the CO2-concentrating mechanism and ciliogenesis. We illustrate the value of this resource by validating phenotypes and gene functions, including three new components of an actin cytoskeleton defense pathway. The data also inform phenotype discovery in land plants; mutants in Arabidopsis thaliana genes exhibit phenotypes similar to those we observed in their Chlamydomonas homologs. We anticipate that this resource will guide the functional characterization of genes across the tree of life. Systematic phenotyping of 58,101 mutants of the model eukaryotic alga Chlamydomonas reinhardtii under 121 environmental and chemical stress conditions provides a large resource for characterizing gene function.
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影响因子：
56.9
作者：
Hillenmeyer, Maureen E.;Fung, Eula;Giaever, Guri
通讯作者：
Giaever, Guri
影响因子：
64.5
作者：
Feldman, RMR;Correll, CC;Deshaies, RJ
通讯作者：
Deshaies, RJ
DOI：
10.1073/pnas.081593498
发表时间：
2001-04-24
影响因子：
11.1
作者：
Fukuzawa, H;Miura, K;Ohyama, K
通讯作者：
Ohyama, K
影响因子：
7.2
作者：
Heitzeberg, F;Chen, IP;Puchta, H
通讯作者：
Puchta, H
影响因子：
14.9
作者：
Garcia, Valerie;Salanoubat, Marcel;Tissier, Alain
通讯作者：
Tissier, Alain