Redox of Fungal Multicopper Oxidase: A Potential Driving Factor for the Silicate Mineral Weathering
Redox of Fungal Multicopper Oxidase: A Potential Driving Factor for the Silicate Mineral Weathering
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真菌多铜氧化酶的氧化还原：硅酸盐矿物风化的潜在驱动因素
DOI：
10.1080/01490451.2018.1485065
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发表时间：
2018-10
影响因子：
2.3
通讯作者：
Lian B
中科院分区：
文献类型：
--
作者：
Wang WY;Sun QB;Lian B
摘要微生物矿物风化在自然界中普遍存在，但与氧化还原酶的直接作用相关的研究（例如，真菌多铜氧化酶）对硅酸盐矿物风化作用的研究很少。本研究采用异源表达和基因过表达的基因工程技术及电化学方法研究了尼日尔曲霉多铜氧化酶（McoA）及其基因（mcoA）在含钾硅酸盐矿物（KBS）风化过程中的作用。在耐KBS实验中，mcoA过表达菌株比野生型菌株具有更强的K+释放能力，异源表达的McoA具有直接耐KBS和释放K+的能力。电化学测试结果表明，McoA的还原电位为0.302 V，使KBS修饰电极的电流增加了一倍，说明McoA在KBS的老化实验中具有增强电传递的作用。本研究证实了A.尼日尔可直接参与硅酸盐矿物的风化，促进矿物不稳定边缘的电子传递，加速矿物风化。该研究将使人们更好地了解氧化还原酶在硅酸盐矿物生物化学风化中的作用，并确定一种可能的方法，通过基因工程操作来提高微生物对矿物的风化能力。
Abstract Microbial mineral weathering is ubiquitous in nature, but research related to the direct effect of oxidoreductase (e.g., fungal multicopper oxidase) on silicate mineral weathering is rare. This study uses genetic engineering technologies of heterologous expression and gene overexpression and electrochemical methods to examine the effects of multicopper oxidase (McoA) and its gene (mcoA) of Aspergillus niger in the weathering of K+-bearing silicate minerals (KBS). In the experiment of weathering KBS, the mcoA-overexpressed strain showed higher K+-release ability than the wild-type strain, and the heterologous-expressed McoA demonstrated a direct potential to weather KBS and release K+. The electrochemical results showed that the reduction potential of McoA was 0.302 V, and doubled the current of the KBS-modified electrode, meaning that McoA can enhance the electricity transfer in the experiment of weathering KBS. This study confirmed that McoA of A. niger can directly participate in the weathering of silicate minerals, which promotes electron transport at the instable edge of minerals to accelerate mineral weathering. The study will enable greater understanding of the role of oxidoreductase in the biochemical weathering of silicate minerals and identify a possible way to improve the microbial weathering capacity on minerals by genetic engineering manipulation.
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影响因子：
2.3
作者：
Leilei Xiao;J. Hao;Weiying Wang;B. Lian;G. Shang;Yunwen Yang;Congqiang Liu;Shijie Wang
通讯作者：
Leilei Xiao;J. Hao;Weiying Wang;B. Lian;G. Shang;Yunwen Yang;Congqiang Liu;Shijie Wang
构巢曲霉应对可溶性钾缺乏的基因表达的整体观察
影响因子：
2.6
作者：
Xiao Leilei;Sun Qibiao;Lian Bin
通讯作者：
Lian Bin
影响因子：
5
作者：
Balogh-Brunstad, Zsuzsanna;Keller, C. Kent;Bormann, Bernard T.
通讯作者：
Bormann, Bernard T.
影响因子：
15
作者：
Li, H;Webb, SP;Jensen, JH
通讯作者：
Jensen, JH
影响因子：
5
作者：
B. Lian;Bin Wang;Mu Pan;Congqiang Liu;H. Teng
通讯作者：
B. Lian;Bin Wang;Mu Pan;Congqiang Liu;H. Teng