Endogenous non-retroviral RNA virus elements in mammalian genomes.
Endogenous non-retroviral RNA virus elements in mammalian genomes.
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逆转录病毒是已知的唯一一种以内源性原病毒的形式留下化石记录的病毒，大约8%的人类基因组由这些元素组成。虽然许多其他病毒，包括非逆转录病毒RNA病毒，已知在复制过程中产生自身基因组的DNA形式，但在动物种系中没有发现DNA。博尔纳病毒是一种非分节的负义RNA病毒，在RNA病毒中是独特的，因为它们在细胞核中建立持续感染，。本研究表明，在几种哺乳动物物种（包括人类、非人灵长类动物、啮齿动物和大象）的基因组中存在与博纳病毒核蛋白(N)基因同源的元素。这些序列被命名为内源性Borna-like N （EBLN）元件。一些灵长类动物ebln含有一个完整的开放阅读框（ORF），并以mRNA的形式表达。系统发育分析表明，ebln似乎是由每个特定动物科的不同插入事件产生的。此外，地松鼠的EBLN是在最近的一次整合事件中形成的，而灵长类动物的EBLN必须在4000多万年前形成。我们还发现，目前的哺乳动物博纳病病毒（BDV）的theNmRNA可以在持续感染的培养细胞的基因组中形成ebln样元件。我们的研究结果为哺乳动物基因组中非逆转录病毒衍生元件的内源性作用提供了第一个证据，不仅为内源性元件的产生提供了新的见解，而且还为博纳病毒在其宿主中作为遗传新颖性来源的作用提供了新的见解。
Retroviruses are the only group of viruses known to have left a fossil record, in the form of endogenous proviruses, and approximately 8% of the human genome is made up of these elements,. Although many other viruses, including non-retroviral RNA viruses, are known to generate DNA forms of their own genomes during replication,,, none has been found as DNA in the germline of animals. Bornaviruses, a genus of non-segmented, negative-sense RNA virus, are unique among RNA viruses in that they establish persistent infection in the cell nucleus,,. Here we show that elements homologous to the nucleoprotein (N) gene of bornavirus exist in the genomes of several mammalian species, including humans, non-human primates, rodents and elephants. These sequences have been designated endogenous Borna-like N (EBLN) elements. Some of the primate EBLNs contain an intact open reading frame (ORF) and are expressed as mRNA. Phylogenetic analyses showed that EBLNs seem to have been generated by different insertional events in each specific animal family. Furthermore, the EBLN of a ground squirrel was formed by a recent integration event, whereas those in primates must have been formed more than 40 million years ago. We also show that theNmRNA of a current mammalian bornavirus, Borna disease virus (BDV), can form EBLN-like elements in the genomes of persistently infected cultured cells. Our results provide the first evidence for endogenization of non-retroviral virus-derived elements in mammalian genomes and give novel insights not only into generation of endogenous elements, but also into a role of bornavirus as a source of genetic novelty in its host.
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影响因子：
11.4
作者：
Maestre, J;Tchenio, T;Heidmann, T
通讯作者：
Heidmann, T
影响因子：
4.4
作者：
MINAMI, M;POUSSIN, K;PATERLINI, P
通讯作者：
PATERLINI, P
影响因子：
5.4
作者：
Chase, Geoffrey;Mayer, Daniel;Schwemmle, Martin
通讯作者：
Schwemmle, Martin
影响因子：
10.7
作者：
SAITOU, N;NEI, M
通讯作者：
NEI, M
DOI：
10.1007/978-1-59745-039-3_4
发表时间：
2006-01-01
期刊：
GENOMIC DISORDERS: THE GENOMIC BASIS OF DISEASE
影响因子：
--
作者：
Pavlicek, Adam;Jurka, Jerzy
通讯作者：
Jurka, Jerzy