Myometrial characteristics of the syrian hamster uterine smooth muscle cell line, SHM
Myometrial characteristics of the syrian hamster uterine smooth muscle cell line, SHM
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叙利亚仓鼠子宫平滑肌细胞系 SHM 的肌层特征
DOI：
10.1007/bf02639382
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
J. Roberts
中科院分区：
文献类型：
--
作者：
R. Riemer;Y. Sadovsky;J. Roberts
我们描述了一种新的平滑肌细胞系SHM（叙利亚仓鼠子宫肌层）的几个特征，该细胞系来源于一只雌性叙利亚（金黄色）仓鼠的原发性子宫平滑肌肉瘤，该肉瘤是由慢性雌激素加雄激素治疗诱导的。为了确定SHM细胞系作为模型的有用性，以了解子宫肌层功能及其调节，我们研究了这些细胞的形态学和免疫细胞化学特性，以及子宫收缩激动剂通过磷酸肌醇水解激活跨膜信号传导的能力。SHM细胞呈梭形，光滑的肌肉样形态时，融合时，更紧凑，星状。与原代肌细胞一样，SHM细胞表达中间丝结蛋白和收缩蛋白α平滑肌肌动蛋白，但不表达上皮抗原细胞角蛋白。去甲肾上腺素和缓激肽刺激子宫收缩和肌醇多磷酸的产生，也刺激SHM细胞肌醇多磷酸的产生。SHM细胞的最大磷脂酰肌醇信号反应低于原代仓鼠子宫肌细胞。我们的结论是，SHM细胞系表现出主要的子宫肌细胞的特点，因此可能是一个有用的系统，通过检查子宫肌层功能的调节机制。
SummaryWe describe several characteristics of a novel smooth muscle cell line, SHM (Syrian hamster myometrium) derived from a primary uterine leiomyosarcoma which was induced by chronic estrogen plus androgen treatment of a female Syrian (golden) hamster. To determine the usefulness of the SHM cell line as a model for understanding myometrial function and its regulation, we have examined the morphologic and immunocytochemical properties of these cells, and the ability of uterotonic agonists to activate transmembrane signaling via phosphoinositide hydrolysis. The SHM cells exhibited a spindle-shape, smooth musclelike morphology when subconfluent, and a more compact, stellate shape at confluence. Like primary myocytes, SHM cells expressed the intermediate filament desmin and the contractile protein alpha smooth muscle actin, but not the epithelial antigen cytokeratin. Norepinephrine and bradykinin, which stimulate contraction and inositol polyphosphate production in the uterus, also stimulated inositol polyphosphate production in SHM cells. The maximal phosphoinositide signaling responses were lower in SHM cells compared with primary hamster uterine myocytes. We conclude that the SHM cell line exhibits primary uterine myocyte characteristics, and may therefore be a useful system for examining the mechanisms through which myometrial functions are regulated.
兔子宫肌细胞中雌激素受体的浓度在培养物中降低。
影响因子：
9.8
作者：
Sadovsky,Y;Riemer,RK;Roberts,JM
通讯作者：
Roberts,JM
雌激素增加兔子宫中肾上腺素能介导的磷酸肌醇的产生，但不增加胆碱能介导的肌醇磷酸的产生。
影响因子：
3.6
作者：
Riemer,RK;Goldfien,A;Roberts,JM
通讯作者：
Roberts,JM
磷酸肌醇在跨膜信号传导中的作用。
影响因子：
33.6
作者：
Rana,RS;Hokin,LE
通讯作者：
Hokin,LE