Salivary proteins associated with hyperglycemia in diabetes: a proteomic analysis.
Salivary proteins associated with hyperglycemia in diabetes: a proteomic analysis.
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DOI：
10.1039/c3mb70196d
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发表时间：
2013-11
影响因子：
--
通讯作者：
Buse JB
中科院分区：
文献类型：
--
作者：
Bencharit S;Baxter SS;Carlson J;Byrd WC;Mayo MV;Border MB;Kohltfarber H;Urrutia E;Howard-Williams EL;Offenbacher S;Wu MC;Buse JB
有效监测血糖水平对于患者更好地控制糖尿病是必要的。然而，只有大约四分之一的糖尿病患者需要密切监测血糖，每天定期检查血糖。患者依从性的潜在障碍之一是血液采样要求。糖尿病患者的唾液及其蛋白质含量可能会改变，这可能是由于血糖控制的变化。我们在这里提出，唾液蛋白质组的糖尿病患者可能是不同的基础上，他们的血糖控制反映在A1 C水平。共招募了153名1型或2型糖尿病受试者。每种类型的糖尿病受试者根据他们的A1 C水平分为5组; <7，7-8，8-9，9-10，>10。为了研究与A1 C相关的整体蛋白质组学变化，使用无标记定量蛋白质组学创建了来自每组的合并唾液样本的蛋白质组学分析。然后对个体受试者（每组N=4）进行类似的蛋白质组学分析，以检查合并样品中可能丰度较低的蛋白质。主成分分析（PCA）和聚类分析（p<0.01和p<0.001）用于定义蛋白质组差异。因此，我们定义了与A1 C变化相关的唾液蛋白质组学变化。这项研究表明，根据A1 C水平，糖尿病受试者的唾液蛋白质组谱存在差异。
Effective monitoring of glucose levels is necessary for patients to achieve greater control over their diabetes. However, only about a quarter of subjects with diabetes who requires close serum glucose monitoring, regularly check their serum glucose daily. One of the potential barriers to patient compliance is the blood sampling requirement. Saliva and its protein contents can be altered in subjects with diabetes, possibly due to changes in glycemic control. We propose here that salivary proteomes of subjects with diabetes may be different based on their glycemic control as reflected in A1C levels. A total of 153 subjects with type 1 or 2 diabetes were recruited. Subjects in each type of diabetes were divided into 5 groups based on their A1C levels; <7, 7–8, 8–9, 9–10, >10. To examine the global proteomic changes associated with A1C, the proteomic profiling of pooled saliva samples from each group was created using label-free quantitative proteomics. Similar proteomic analysis for individual subjects (N=4, for each group) were then applied to examine proteins that may be less abundant in pooled samples. Principle component analysis (PCA) and cluster analysis (p<0.01 and p<0.001) were used to define the proteomic differences. We, therefore, defined the salivary proteomic changes associated with A1C changes. This study demonstrates that differences exist between salivary proteomic profiles in subjects with diabetes based on the A1C levels.
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影响因子：
16.2
作者：
Cheng YJ;Gregg EW;Geiss LS;Imperatore G;Williams DE;Zhang X;Albright AL;Cowie CC;Klein R;Saaddine JB
通讯作者：
Saaddine JB
影响因子：
7.7
作者：
Fernández-Real, JM;Peñarroja, G;Ricart, W
通讯作者：
Ricart, W
影响因子：
3.5
作者：
DOLHOFER, R;WIELAND, OH
通讯作者：
WIELAND, OH
影响因子：
7.7
作者：
Engström, G;Hedblad, B;Lindgärde, F
通讯作者：
Lindgärde, F
影响因子：
8.2
作者：
Hertle, E.;van Greevenbroek, M. M. J.;Stehouwer, C. D. A.
通讯作者：
Stehouwer, C. D. A.