Pain-related anxiety-like behavior requires CRF1 receptors in the amygdala.
Pain-related anxiety-like behavior requires CRF1 receptors in the amygdala.
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DOI：
10.1186/1744-8069-3-13
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发表时间：
2007-06-05
期刊：
影响因子：
3.3
通讯作者：
Neugebauer, Volker
中科院分区：
文献类型：
--
作者：
Ji, Guangchen;Fu, Yu;Ruppert, Katherine A.;Neugebauer, Volker
促肾上腺皮质激素释放因子受体CRF1与焦虑和抑郁的神经生物学机制有关。杏仁核在情感状态和焦虑、抑郁等疾病中起着重要作用。杏仁核也逐渐成为疼痛影响的神经基质。然而，杏仁核在疼痛和焦虑的相互作用中的作用仍有待确定。这项研究验证了杏仁核中的CRF1受体与疼痛相关焦虑密切相关的假设。采用升高+迷宫（EPM）法测定成年雄性大鼠的焦虑样行为。测量了张开手臂的偏好（张开手臂进入者与进入者总数的比例）。通过测量膝关节有害机械刺激的后肢退缩阈值来评估有害行为。对正常大鼠和关节内注射高岭土/卡拉胶引起的单膝关节炎大鼠进行测量。选择性CRF1受体拮抗剂（NBI27914）或载体被系统（i.p）或进入杏仁核中央核（CeA，通过微透析）。关节炎组在EPM中对张开双臂的偏好降低，后肢戒断阈值降低。全身或杏仁核内（进入CeA）给予NBI27914，而不是对照，抑制焦虑样行为和有害疼痛反应，几乎逆转关节炎疼痛相关的变化。这项研究首次表明，杏仁核中的CRF1受体对关节炎疼痛模型中疼痛相关的焦虑样行为和有害反应起着至关重要的作用。结果是一个直接的证明，临床充分证明疼痛和焦虑之间的关系涉及杏仁核。
Corticotropin-releasing factor receptor CRF1 has been implicated in the neurobiological mechanisms of anxiety and depression. The amygdala plays an important role in affective states and disorders such as anxiety and depression. The amygdala is also emerging as a neural substrate of pain affect. However, the involvement of the amygdala in the interaction of pain and anxiety remains to be determined. This study tested the hypothesis that CRF1 receptors in the amygdala are critically involved in pain-related anxiety. Anxiety-like behavior was determined in adult male rats using the elevated plus maze (EPM) test. The open-arm preference (ratio of open arm entries to the total number of entries) was measured. Nocifensive behavior was assessed by measuring hindlimb withdrawal thresholds for noxious mechanical stimulation of the knee. Measurements were made in normal rats and in rats with arthritis induced in one knee by intraarticular injections of kaolin/carrageenan. A selective CRF1 receptor antagonist (NBI27914) or vehicle was administered systemically (i.p.) or into the central nucleus of the amygdala (CeA, by microdialysis). The arthritis group showed a decreased preference for the open arms in the EPM and decreased hindlimb withdrawal thresholds. Systemic or intraamygdalar (into the CeA) administration of NBI27914, but not vehicle, inhibited anxiety-like behavior and nocifensive pain responses, nearly reversing the arthritis pain-related changes. This study shows for the first time that CRF1 receptors in the amygdala contribute critically to pain-related anxiety-like behavior and nocifensive responses in a model of arthritic pain. The results are a direct demonstration that the clinically well-documented relationship between pain and anxiety involves the amygdala.
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影响因子：
5.3
作者：
Han, JS;Li, WD;Neugebauer, V
通讯作者：
Neugebauer, V
影响因子：
5.3
作者：
Baliki, Marwan N.;Chialvo, Dante R.;Apkarian, A. Vania
通讯作者：
Apkarian, A. Vania
影响因子：
3.3
作者：
Han JS;Fu Y;Bird GC;Neugebauer V
通讯作者：
Neugebauer V
影响因子：
6.7
作者：
Charney, DS
通讯作者：
Charney, DS
影响因子：
3
作者：
Han, JS;Bird, GC;Neugebauer, V
通讯作者：
Neugebauer, V