Horizontal gene transfer and ecological interactions jointly control microbiome stability.
Horizontal gene transfer and ecological interactions jointly control microbiome stability.
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DOI：
10.1371/journal.pbio.3001847
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发表时间：
2022-11
期刊：
影响因子：
9.8
通讯作者：
中科院分区：
文献类型：
--
作者：
编码对压力源（如抗生素或环境污染物）的抗性的基因在微生物组中广泛存在，通常编码在移动的遗传元件上。然而，尽管它们普遍存在，但耐药基因及其流动性对微生物群落动态的影响在很大程度上仍然未知。在这里，我们发展生态进化理论，探索抗性基因如何改变不同微生物组的稳定性，以应对压力。我们表明，将抗性基因添加到微生物组通常会增加其整体稳定性，特别是对于具有高转移率的移动的遗传元件上的基因，这些基因可以有效地在整个社区传播抗性。然而，抗性基因对个体分类群稳定性的影响根据个体分类群的身份、抗性基因的移动性和群落内生态相互作用的网络而显著不同。非移动抗性基因可以使合作群落中的易感类群受益，但却损害竞争群落中的易感类群。此外，虽然移动的抗性基因的转移一般增加了以前易受干扰的受体类群的稳定性，它可以降低原来的抗性供体taxon.We的稳定性证实了关键的理论预测实验使用竞争性土壤微宇宙社区。在这里，通过添加接合质粒上编码的移动的抗性基因，增加了易感微生物群落对扰动的稳定性，但当这些相同的基因在染色体上编码时，稳定性降低。总之，这些发现突出了生态相互作用和水平基因转移之间的相互作用在驱动不同微生物组的生态进化动力学方面的重要性。耐药基因及其迁移性对微生物群落的动态有何影响？这项研究开发和测试了一种新的生态进化理论，以探索生态相互作用和水平基因转移如何结合联合收割机来塑造微生物组的稳定性。
Genes encoding resistance to stressors, such as antibiotics or environmental pollutants, are widespread across microbiomes, often encoded on mobile genetic elements. Yet, despite their prevalence, the impact of resistance genes and their mobility upon the dynamics of microbial communities remains largely unknown. Here we develop eco-evolutionary theory to explore how resistance genes alter the stability of diverse microbiomes in response to stressors. We show that adding resistance genes to a microbiome typically increases its overall stability, particularly for genes on mobile genetic elements with high transfer rates that efficiently spread resistance throughout the community. However, the impact of resistance genes upon the stability of individual taxa varies dramatically depending upon the identity of individual taxa, the mobility of the resistance gene, and the network of ecological interactions within the community. Nonmobile resistance genes can benefit susceptible taxa in cooperative communities yet damage those in competitive communities. Moreover, while the transfer of mobile resistance genes generally increases the stability of previously susceptible recipient taxa to perturbation, it can decrease the stability of the originally resistant donor taxon. We confirmed key theoretical predictions experimentally using competitive soil microcosm communities. Here the stability of a susceptible microbial community to perturbation was increased by adding mobile resistance genes encoded on conjugative plasmids but was decreased when these same genes were encoded on the chromosome. Together, these findings highlight the importance of the interplay between ecological interactions and horizontal gene transfer in driving the eco-evolutionary dynamics of diverse microbiomes. What is the impact of resistance genes and their mobility on the dynamics of microbial communities? This study develops and tests a new body of eco-evolutionary theory to explore how ecological interactions and horizontal gene transfer combine to shape microbiome stability.
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DOI：
10.1073/pnas.1600974113
发表时间：
2016-07-19
影响因子：
11.1
作者：
Hall, James P. J.;Wood, A. Jamie;Brockhurst, Michael A.
通讯作者：
Brockhurst, Michael A.
影响因子：
5.1
作者：
Lambertsen, L;Sternberg, C;Molin, S
通讯作者：
Molin, S
中国土壤微生物分类和功能属性对汞污染的一致响应
影响因子：
15.5
作者：
Liu YR;Delgado-Baquerizo M;Bi L;Zhu J;He JZ
通讯作者：
He JZ
影响因子：
56.9
作者：
Coyte, Katharine Z.;Schluter, Jonas;Foster, Kevin R.
通讯作者：
Foster, Kevin R.
影响因子：
64.8
作者：
Bell, T;Newman, JA;Lilley, AK
通讯作者：
Lilley, AK