Picroscope: low-cost system for simultaneous longitudinal biological imaging.
Picroscope: low-cost system for simultaneous longitudinal biological imaging.
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DOI：
10.1038/s42003-021-02779-7
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发表时间：
2021-11-04
影响因子：
5.9
通讯作者：
Teodorescu M
中科院分区：
文献类型：
--
作者：
Ly VT;Baudin PV;Pansodtee P;Jung EA;Voitiuk K;Rosen YM;Willsey HR;Mantalas GL;Seiler ST;Selberg JA;Cordero SA;Ross JM;Rolandi M;Pollen AA;Nowakowski TJ;Haussler D;Mostajo-Radji MA;Salama SR;Teodorescu M
跨多个条件和复制的同时纵向成像对于旨在了解生物过程和疾病的科学研究至关重要。然而，对于世界上大多数学术和教学实验室来说，能够完成这些任务的成像系统在经济上是无法实现的。在这里，我们提出了Picroscope，这是第一个低成本的同时纵向生物成像系统，主要使用现成的和3d打印材料。Picroscope与标准的24孔细胞培养板兼容，并捕获3D z堆叠图像数据。Picroscope可以远程控制，允许自动成像，研究者的干预最小。在这里，我们在一系列应用中使用这个系统。我们收集了蛙类、斑马鱼和涡虫的纵向整体生物图像数据。我们还在培养箱内收集图像数据，观察二维单层和三维哺乳动物组织培养模型。使用这个工具，我们可以在很长一段时间内测量整个生物体或单个细胞的行为。Ly等人报道了一种多井成像系统（Picroscope），该系统可以同时对24口井进行纵向亮场z叠加成像。他们通过对斑马鱼胚胎、涡虫、热带爪蟾在28小时内的发育以及培养箱内的胚胎干细胞单层和类器官进行成像来证明其能力。
Simultaneous longitudinal imaging across multiple conditions and replicates has been crucial for scientific studies aiming to understand biological processes and disease. Yet, imaging systems capable of accomplishing these tasks are economically unattainable for most academic and teaching laboratories around the world. Here, we propose the Picroscope, which is the first low-cost system for simultaneous longitudinal biological imaging made primarily using off-the-shelf and 3D-printed materials. The Picroscope is compatible with standard 24-well cell culture plates and captures 3D z-stack image data. The Picroscope can be controlled remotely, allowing for automatic imaging with minimal intervention from the investigator. Here, we use this system in a range of applications. We gathered longitudinal whole organism image data for frogs, zebrafish, and planaria worms. We also gathered image data inside an incubator to observe 2D monolayers and 3D mammalian tissue culture models. Using this tool, we can measure the behavior of entire organisms or individual cells over long-time periods. Ly et al. report a multi-well imaging system (the Picroscope) that can perform longitudinal bright-field z-stack imaging for 24 wells simultaneously. They demonstrate its capability by imaging zebrafish embryos, planaria worms, development of Xenopus tropicalis over a 28-h period and embryonic stem cell monolayer and organoids inside an incubator.
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影响因子：
9.8
作者：
Baden T;Chagas AM;Gage GJ;Marzullo TC;Prieto-Godino LL;Euler T
通讯作者：
Euler T
DOI：
10.1107/s2053230x18016515
发表时间：
2018-12-01
影响因子：
0.9
作者：
Bohm, Andrew
通讯作者：
Bohm, Andrew
DOI：
10.1073/pnas.1608198113
发表时间：
2016-10-18
影响因子：
11.1
作者：
Almassalha, Luay M.;Bauer, Greta M.;Backman, Vadim
通讯作者：
Backman, Vadim
影响因子：
4.6
作者：
Abe-Fukasawa N;Otsuka K;Aihara A;Itasaki N;Nishino T
通讯作者：
Nishino T
影响因子：
3.7
作者：
Gonzalez-Gonzalez, Everardo;Trujillo-de Santiago, Grissel;Moises Alvarez, Mario
通讯作者：
Moises Alvarez, Mario