Tyrosine phosphorylation pathway is involved in interferon‐gamma (IFN‐γ) production; effect of sodium ortho vanadate
Tyrosine phosphorylation pathway is involved in interferon‐gamma (IFN‐γ) production; effect of sodium ortho vanadate
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酪氨酸磷酸化途径参与原钒酸钠的干扰素-γ (IFN-γ) 产生作用；
DOI：
10.1111/j.1365-2249.1995.tb03618.x
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发表时间：
1995
影响因子：
4.6
通讯作者：
F. Pandolfi
中科院分区：
文献类型：
--
作者：
A. Giovannetti;A. Aiuti;P. Pizzoli;M. Pierdominici;E. Agostini;A. Oliva;F. Dianzani;F. Aiuti;F. Pandolfi
The molecular mechanisms regulating IFN‐γ production have yet to be well characterized. We describe here how treatment of activated cultures of peripheral blood mononuclear cells (PBMC) with the phosphotyrosine phosphatases (PTP) inhibitor sodium ortho vanadate results in greatly enhanced IFN‐γ production. Conversely, cellular proliferation of the same cultures is profoundly inhibited by treatment with vanadate, while the expression of IL‐2R and DR molecules on activated lymphocytes remains substantially unmodified. Increased IFN‐γ production, but not inhibition of cellular proliferation, was also observed in mitogen‐activated vanadate‐treated Jurkat cells. On the other hand, IFN‐γ production induced in cultures of PBMC treated or not with vanadate, was strongly inhibited by incubation with the protein tyrosine kinase (PTK) inhibitor herbimycin A. As a result of the inhibited phosphatase activity, substrates for PTK become hyperphosphorylated on tyrosine residues, as shown by Western blot analysis of cell lysates from cultures of PBMC treated with vanadate. We suggest that the tyrosine phosphorylation pathway plays a role in regulating IFN‐γ production.
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DOI：
10.1089/jir.1988.8.527
发表时间：
1988
期刊：
Journal of interferon research
影响因子：
--
作者：
Young,HA;Birchenall-Sparks,M;Kovacs,E;Dorman,L;Ruscetti,FW
通讯作者：
Ruscetti,FW
影响因子：
4.4
作者：
H. Fox;B. Bond;T. Parslow
通讯作者：
H. Fox;B. Bond;T. Parslow
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DOI：
--
发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Reem,GH;Carding,S;Reinherz,EL
通讯作者：
Reinherz,EL
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DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Swarup,G;SpeegJr,KV;Cohen,S;Garbers,DL
通讯作者：
Garbers,DL
人类 T 细胞亚群的血清学和功能表征。
影响因子：
4.6
作者：
Pandolfi,F;Strong,DM;Slease,RB;Bonnard,GD
通讯作者：
Bonnard,GD