Identification of a Prunus MAX1 Homolog as a Unique Strigol Synthase from Carlactone Bypassing 5-Deoxystrigol
Identification of a Prunus MAX1 Homolog as a Unique Strigol Synthase from Carlactone Bypassing 5-Deoxystrigol
复制标题
鉴定 Prunus MAX1 同源物为绕过 5-Deoxystrigol 的 Carlactone 的独特 Strigol 合酶
DOI：
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Yanran Li
中科院分区：
文献类型：
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作者：
Sheng Wu;Anqi Zhou;Kozue Hiugano;Akiyoshi Yoda;Xiaonan Xie;Kenji Yamane;K. Miura;T. Nomura;Yanran Li
独脚金醇是第一个被发现的独脚金内酯（SL），但生物合成途径仍然难以捉摸。在这里，通过使用微生物SL生产平台进行快速基因筛选，我们在功能上鉴定了李属中的独脚金合酶（PpMAX 1c，一种细胞色素P450 711 A酶），该合酶通过催化多步氧化和C环化直接从SL前体卡内酯合成独脚金，绕过5-脱氧独脚金醇的合成。通过对烟草中独脚金醇生物合成的改造，验证了PpMAX 1c的功能。另外的基因组分析和功能验证证实，桃还编码列当醇合酶（PpCYP 722 C，细胞色素P450 722 C酶），这暗示在桃中存在独脚金型SL和列当醇型SL，并且通过桃幼苗的代谢分析证实。这项工作突出了在很大程度上未开发的CYP 711 A同系物家族的催化多样性，并设置了基础，以表征不同类型的SL在经济上重要的李的作用。
Strigol was the first strigolactone (SL) to be discovered, but the biosynthetic pathway remains elusive. Here, through rapid gene screening using a microbial SL-producing platform, we functionally identified a strigol synthase (PpMAX1c, a cytochrome P450 711A enzyme) in Prunus that synthesizes strigol directly from the SL precursor carlactone through catalyzing multi-step oxidations and C-ring cyclization, bypassing the synthesis of 5-deoxystrigol. The function of PpMAX1c was validated through reconstructing the biosynthesis of strigol in Nicotiana benthamiana. Additional genomic analysis and functional verification confirm that peach also encodes an orobanchol synthase (PpCYP722C, a cytochrome P450 722C enzyme), which hints at the presence of both strigol-type and orobanchol-type SLs in peach and was confirmed through metabolic analysis of peach seedlings. This work highlights the catalytic diversity of the largely unexplored family of CYP711A homologs and sets the foundation to characterize the roles of different types of SLs in the economically important Prunus.
影响因子：
56.9
作者：
Alder, Adrian;Jamil, Muhammad;Al-Babili, Salim
通讯作者：
Al-Babili, Salim
影响因子：
13.6
作者：
Wu S;Ma X;Zhou A;Valenzuela A;Zhou K;Li Y
通讯作者：
Li Y