Nature and distribution of large sequence polymorphisms in Saccharomyces cerevisiae.
Nature and distribution of large sequence polymorphisms in Saccharomyces cerevisiae.
复制标题
酿酒酵母中大序列多态性的性质和分布。
DOI：
10.1111/j.1567-1364.2011.00748.x
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发表时间：
2011-11
影响因子：
3.2
通讯作者：
McCusker JH
中科院分区：
文献类型：
--
作者：
Muller LA;McCusker JH
为了更好地了解酿酒酵母中大序列多态性（LSPs）的全基因组分布和性质，我们对88株单倍体或纯合二倍体酿酒酵母的基因组DNA进行了杂交。酿酒酵母菌株的不同地理来源和源基板上的高密度平铺阵列。基于杂交丢失，我们鉴定了384个大于500 bp的LSP，它们位于基因组的188个非重叠区域。通过聚合酶链反应扩增和/或DNA测序的验证显示，39个LSP是由于缺失，而74个LSP涉及的序列偏离足够远，从S288 c参考基因组序列，以防止杂交到微阵列功能。LSP位置偏向于染色体的亚端粒区域，在那里，参与运输或发酵的基因的高度遗传变异被认为有助于S.新环境下的酿酒厂分离的LSP序列似乎具有不同的等位基因祖先，并且在许多情况下被鉴定为S。paradoxus渐渗。
To obtain a better understanding of the genome-wide distribution and the nature of large sequence polymorphisms (LSPs) in Saccharomyces cerevisiae, we hybridized genomic DNA of 88 haploid or homozygous diploid S. cerevisiae strains of diverse geographic origins and source substrates onto high-density tiling arrays. Based on loss of hybridization, we identified 384 LSPs larger than 500 bp that were located in 188 non-overlapping regions of the genome. Validation by polymerase chain reaction-amplification and/or DNA sequencing revealed that 39 LSPs were due to deletions, while 74 LSPs involved sequences diverged far enough from the S288c reference genome sequence as to prevent hybridization to the microarray features. The LSP locations were biased towards the subtelomeric regions of chromosomes, where high genetic variation in genes involved in transport or fermentation is thought to facilitate rapid adaptation of S. cerevisiae to new environments. The diverged LSP sequences appear to have different allelic ancestries and were in many cases identified as S. paradoxus introgressions.
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全基因组对酿酒酵母菌株的核苷酸水平变异的分析。
影响因子：
3.7
作者：
Schacherer J;Ruderfer DM;Gresham D;Dolinski K;Botstein D;Kruglyak L
通讯作者：
Kruglyak L
影响因子：
64.8
作者：
Kellis, M;Patterson, N;Lander, ES
通讯作者：
Lander, ES
影响因子：
4.8
作者：
Miyake, T;Hiraishi, H;Ono, BI
通讯作者：
Ono, BI
影响因子：
64.8
作者：
Schacherer J;Shapiro JA;Ruderfer DM;Kruglyak L
通讯作者：
Kruglyak L
影响因子：
56.9
作者：
Gresham, D;Ruderfer, DM;Kruglyak, L
通讯作者：
Kruglyak, L