Orthogonally oriented scaffolds with aligned fibers for engineering intestinal smooth muscle.
Orthogonally oriented scaffolds with aligned fibers for engineering intestinal smooth muscle.
复制标题
DOI：
10.1016/j.biomaterials.2015.05.023
复制
发表时间：
2015-08
期刊：
影响因子：
14
通讯作者：
Dunn, James C. Y.
中科院分区：
文献类型：
--
作者：
Kobayashi, Masae;Lei, Nan Ye;Wang, Qianqian;Wu, Benjamin M.;Dunn, James C. Y.
控制细胞排列在工程化具有所需结构和功能的肠道中至关重要。虽然以前的研究已经检查了细胞在平行纤维表面（x-y平面）上的定向排列，但尚未报道具有定向纤维的植入支架内的细胞排列的定量分析。本研究检查了由两层正交取向纤维制成的支架在x-z和y-z平面中的细胞排列。用免疫荧光法评估植入支架内的细胞取向。细胞取向和纤维方向之间的相干性的定量分析证实，细胞不仅在表面（x-y平面）上而且在支架内部（x-z和y-z平面）沿纤维沿着。我们的研究表明，两层正交排列的支架可以产生类似于肠环形和纵向平滑肌的细胞组织学组织。
Controlling cellular alignment is critical in engineering intestines with desired structure and function. Although previous studies have examined the directional alignment of cells on the surface (x-y plane) of parallel fibers, quantitative analysis of the cellular alignment inside implanted scaffolds with oriented fibers has not been reported. This study examined the cellular alignment in the x-z and y-z planes of scaffolds made with two layers of orthogonally oriented fibers. The cellular orientation inside implanted scaffolds was evaluated with immunofluorescence. Quantitative analysis of coherency between cell orientation and fiber direction confirmed that cells aligned along the fibers not only on the surface (x-y plane) but also inside the scaffolds (x-z & y-z planes). Our study demonstrated that two layers of orthogonally aligned scaffolds can generate the histological organization of cells similar to that of intestinal circular and longitudinal smooth muscle.
登录
查看更多内容
影响因子：
29.4
作者：
Bitar KN;Raghavan S;Zakhem E
通讯作者：
Zakhem E
DOI：
10.1016/j.giec.2014.04.002
发表时间：
2014-07-01
影响因子：
--
作者：
Coviello, Lisa C;Stein, Sharon L
通讯作者：
Stein, Sharon L
影响因子：
4.9
作者：
Hanauer, SB
通讯作者：
Hanauer, SB
影响因子：
3.5
作者：
Reneker, DH;Chun, I
通讯作者：
Chun, I
影响因子：
37.8
作者：
Pektok, Erman;Nottelet, Benjamin;Walpoth, Beat H.
通讯作者：
Walpoth, Beat H.